Construct: ORF TRCN0000474341
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000507.1_s317c1
- Derived from:
- ccsbBroadEn_13460
- DNA Barcode:
- GTTCCAGTTGCCGCGCCGGGGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAALADL2 (254827)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474341
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_011512617.3 | 50.3% | 47.7% | (many diffs) |
| 2 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006082.2 | 45.3% | 42.9% | (many diffs) |
| 3 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006081.2 | 40.8% | 38.7% | (many diffs) |
| 4 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_011512615.3 | 40.7% | 38.7% | (many diffs) |
| 5 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_006713560.3 | 39.6% | 37.6% | (many diffs) |
| 6 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006070.2 | 39.6% | 37.6% | (many diffs) |
| 7 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006071.2 | 39.6% | 37.6% | (many diffs) |
| 8 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006072.2 | 39.6% | 37.6% | (many diffs) |
| 9 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006073.2 | 39.6% | 37.6% | (many diffs) |
| 10 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006074.2 | 39.6% | 37.6% | (many diffs) |
| 11 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006075.2 | 39.6% | 37.6% | (many diffs) |
| 12 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006076.2 | 39.6% | 37.6% | (many diffs) |
| 13 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006077.2 | 39.6% | 37.6% | (many diffs) |
| 14 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006078.2 | 39.6% | 37.6% | (many diffs) |
| 15 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006079.2 | 39.6% | 37.6% | (many diffs) |
| 16 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006080.2 | 39.6% | 37.6% | (many diffs) |
| 17 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_011512613.2 | 39.1% | 37.2% | (many diffs) |
| 18 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | NM_207015.3 | 38.8% | 36.8% | (many diffs) |
| 19 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_011512612.3 | 38.3% | 36.4% | (many diffs) |
| 20 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_011512616.3 | 19.5% | 17.5% | (many diffs) |
| 21 | human | 254827 | NAALADL2 | N-acetylated alpha-linked a... | XM_017006083.2 | 17.2% | 15.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1026
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctatcagaag gtccatgcag atcaaagagc tccaggacac tcacagtact 121 tagacaatga tgaccttcaa gccactgccc ttgacttaga gtgggacatg gagaaggaac 181 tagaggagtc tggttttgac caattccagc tagacagtgc tgagaatcag aacctagggc 241 attcagagac tatagacctc aatcttgatt ccattcaacc agcaacttca cccaaaggaa 301 ggttccagag acttcaagaa gaatctgact acattaccca ttatacacga tctgcaccaa 361 agagcaatcg ctgcaacttt tgccacgtct taaaaatgct ttgcacagcc accattttat 421 ttatttttgg gattttgata ggttattatg tacatacaaa ttgcccttca gatgctccat 481 cttcaggaac agttgatcct cagttatatc aagagattct caagacaatc caggcagaag 541 atattaagaa gtctttcaga aatttggtac aactatataa aaatgaagat gacatggaaa 601 tttcaaagaa gattaagacT CAGTGGACCT CTTTGGGCCT AGAAGATGTA CAGTTTGTAA 661 ATTACTCTGT GCTGCTTGAT CTGCCAGGCC CTTCTCCCAG CACTGTGACT CTGAGCAGCA 721 GTGGTCAATG CTTTCATCCT AATGGCCAGC CTTGCAGTGA AGAAGCCAGA AAAGATAGCA 781 GCCAAGACCT GCTCTATTCA TATGCAGCCT ATTCTGCCAA AGGAACTCTC AAGGCTGAAG 841 TCATCGATGT GAGTTATGGA ATGGCAGATG ATTTAAAAAG GATTAGGAAA ATAAAAAACG 901 TAACAAATCA GATCGCACTC CTGAAATTAG GAAAATTGCC ACTGCTTTAT AAGGTTGGTC 961 CAGTGAATGT TATTCAGTGG TTTGGTCAAT ATTTTGCCTT GTTTTGTTGG AATTATATGC 1021 TTTTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTTCCAGTT GCCGCGCCGG GGTGCACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt