Construct: ORF TRCN0000474352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001957.1_s317c1
- Derived from:
- ccsbBroadEn_01304
- DNA Barcode:
- GGGTTTAATCACTCTCGTTGCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSEN1 (5663)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5663 | PSEN1 | presenilin 1 | NM_007318.3 | 100% | 100% | |
| 2 | human | 5663 | PSEN1 | presenilin 1 | XM_005267866.2 | 100% | 100% | |
| 3 | human | 5663 | PSEN1 | presenilin 1 | XM_011536973.2 | 100% | 100% | |
| 4 | human | 5663 | PSEN1 | presenilin 1 | XM_011536974.2 | 100% | 100% | |
| 5 | human | 5663 | PSEN1 | presenilin 1 | NM_000021.4 | 99.1% | 99.1% | 76_87del |
| 6 | human | 5663 | PSEN1 | presenilin 1 | XM_005267864.3 | 99.1% | 99.1% | 76_87del |
| 7 | human | 5663 | PSEN1 | presenilin 1 | XM_011536972.2 | 99.1% | 99.1% | 76_87del |
| 8 | mouse | 19164 | Psen1 | presenilin 1 | NM_008943.2 | 86.6% | 92% | (many diffs) |
| 9 | mouse | 19164 | Psen1 | presenilin 1 | XM_006515605.3 | 86.6% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1455
- ORF length:
- 1389
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agagttacct gcaccgttgt cctacttcca gaatgcacag atgtctgagg 121 acaaccacct gagcaatact aatgacaata gagaacggca ggagcacaac gacagacgga 181 gccttggcca ccctgagcca ttatctaatg gacgacccca gggtaactcc cggcaggtgg 241 tggagcaaga tgaggaagaa gatgaggagc tgacattgaa atatggcgcc aagcatgtga 301 tcatgctctt tgtccctgtg actctctgca tggtggtggt cgtggctacc attaagtcag 361 tcagctttta tacccggaag gatgggcagc taatctatac cccattcaca gaagataccg 421 agactgtggg ccagagagcc ctgcactcaa ttctgaatgc tgccatcatg atcagtgtca 481 ttgttgtcat gactatcctc ctggtggttc tgtataaata caggtgctat aaggtcatcc 541 atgcctggct tattatatca tctctattgt tgctgttctt tttttcattc atttacttgg 601 gggaagtgtt taaaacctat aacgttgctg tggactacat tactgttgca ctcctgatct 661 ggaattttgg tgtggtggga atgatttcca ttcactggaa aggtccactt cgactccagc 721 aggcatatct cattatgatt agtgccctca tggccctggt gtttatcaag tacctccctg 781 aatggactgc gtggctcatc ttggctgtga tttcagtata tgatttagtg gctgttttgt 841 gtccgaaagg tccacttcgt atgctggttg aaacagctca ggagagaaat gaaacgcttt 901 ttccagctct catttactcc tcaacaatgg tgtggttggt gaatatggca gaaggagacc 961 cggaagctca aaggagagta tccaaaaatt ccaagtataa tgcagaaagc acagaaaggg 1021 agtcacaaga cactgttgca gagaatgatg atggcgggtt cagtgaggaa tgggaagccc 1081 agagggacag tcatctaggg cctcatcgct ctacacctga gtcacgagct gctgtccagg 1141 aactttccag cagtatcctc gctggtgaag acccagagga aaggggagta aaacttggat 1201 tgggagattt cattttctac agtgttctgg ttggtaaagc ctcagcaaca gccagtggag 1261 actggaacac aaccatagcc tgtttcGTAG CCATATTAAT TGGTTTGTGC CTTACATTAT 1321 TACTCCTTGC CATTTTCAAG AAAGCATTGC CAGCTCTTCC AATCTCCATC ACCTTTGGGC 1381 TTGTTTTCTA CTTTGCCACA GATTATCTTG TACAGCCTTT TATGGACCAA TTAGCATTCC 1441 ATCAATTTTA TATCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1501 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1561 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGGTTTAATC ACTCTCGTTG 1621 CGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt