Construct: ORF TRCN0000474353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004594.1_s317c1
- Derived from:
- ccsbBroadEn_09692
- DNA Barcode:
- TCCCATACCAACACTCTCAAGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPM1K (152926)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474353
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | NM_152542.5 | 99.9% | 99.7% | 282T>G |
| 2 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_006714111.4 | 99.9% | 99.7% | 282T>G |
| 3 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007803.2 | 99.9% | 99.7% | 282T>G |
| 4 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007804.2 | 64.7% | 49.7% | (many diffs) |
| 5 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007806.2 | 64.7% | 49.7% | (many diffs) |
| 6 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007805.2 | 62.3% | 49.5% | (many diffs) |
| 7 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007807.2 | 61% | 50.2% | (many diffs) |
| 8 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_005262775.4 | 50.3% | 47.8% | (many diffs) |
| 9 | mouse | 243382 | Ppm1k | protein phosphatase 1K (PP2... | NM_175523.4 | 87.6% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aacagctgcc ttaattactt tggtcagaag tggtgggaac caggtgagaa 121 ggagagtgct gctaagctcc cgcctgctgc aggacgacag gcgggtgaca cccacgtgcc 181 acagctccac ttcagagcct aggtgttctc ggtttgaccc agatggtagt gggagtccag 241 ctacctggga caattttggg atctgggata accgcattga tgagccaatt ctgctgccac 301 ccagcattaa gtatggcaag ccaattccca aaatcagctt ggaaaaggtg gggtgcgcct 361 cacagattgg caaacggaaa gagaatgaag atcggtttga cttcgctcag ctgacagatg 421 aggtcctgta ctttgcagtg tatgatggac acggtggacc tgcagcagct gatttctgtc 481 atacccacat ggagaaatgt attatggatt tgcttcctaa ggagaagaac ttggaaactc 541 tgttgacctt ggcttttcta gaaatagata aagccttttc gagtcatgcc cgcctgtctg 601 ctgatgcaac tcttctgacc tctgggacta ctgcaacagt agccctATTG CGAGATGGTA 661 TTGAACTGGT TGTAGCCAGT GTTGGGGACA GCCGGGCTAT TTTGTGTAGA AAAGGAAAAC 721 CCATGAAGCT GACCATTGAC CATACTCCAG AAAGAAAAGA TGAAAAAGAA AGGATCAAGA 781 AATGTGGTGG TTTTGTAGCT TGGAATAGTT TGGGGCAGCC TCACGTAAAT GGCAGGCTTG 841 CAATGACAAG AAGTATTGGA GATTTGGACC TTAAGACCAG TGGTGTCATA GCAGAACCTG 901 AAACTAAGAG GATTAAGTTA CATCATGCTG ATGACAGCTT CCTGGTCCTC ACCACAGATG 961 GAATTAACTT CATGGTGAAT AGTCAAGAGA TTTGTGACTT TGTCAATCAG TGCCATGATC 1021 CCAACGAAGC AGCCCATGCG GTGACTGAAC AGGCAATACA GTACGGTACT GAGGATAACA 1081 GTACTGCAGT AGTAGTGCCT TTTGGTGCCT GGGGAAAATA TAAGAACTCT GAAATCAACT 1141 TCTCATTCAG CAGAAGCTTT GCCTCCAGTG GACGATGGGC CTGCCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGATCC CATACCAACA CTCTCAAGCC CACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t