Construct: ORF TRCN0000474370
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015260.1_s317c1
- Derived from:
- ccsbBroadEn_08701
- DNA Barcode:
- AGTTTATTACCGTTTACCAAGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPC7 (57113)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474370
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57113 | TRPC7 | transient receptor potentia... | NM_020389.2 | 99.9% | 100% | 501C>T |
| 2 | human | 57113 | TRPC7 | transient receptor potentia... | NM_001167577.1 | 92.8% | 92.9% | 501C>T;777_778ins183 |
| 3 | human | 57113 | TRPC7 | transient receptor potentia... | NM_001167576.1 | 86.5% | 86.5% | 501C>T;777_778ins348 |
| 4 | mouse | 26946 | Trpc7 | transient receptor potentia... | NM_012035.3 | 90.4% | 98.1% | (many diffs) |
| 5 | mouse | 26946 | Trpc7 | transient receptor potentia... | NM_001302372.1 | 90.3% | 98% | (many diffs) |
| 6 | mouse | 26946 | Trpc7 | transient receptor potentia... | XM_006517262.3 | 75.5% | 82.3% | (many diffs) |
| 7 | mouse | 26946 | Trpc7 | transient receptor potentia... | XM_006517263.3 | 75% | 81.7% | (many diffs) |
| 8 | mouse | 26946 | Trpc7 | transient receptor potentia... | XM_006517264.3 | 69.4% | 76% | (many diffs) |
| 9 | mouse | 26946 | Trpc7 | transient receptor potentia... | XR_001780787.1 | 60.5% | (many diffs) | |
| 10 | mouse | 26946 | Trpc7 | transient receptor potentia... | NM_001302373.1 | 48.8% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2655
- ORF length:
- 2586
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgaggaac agcaccttca aaaacatgca gcgccggcac acaacgctga 121 gggagaaggg ccgtcgccag gccatccggg gtcccgccta catgttcaac gagaagggca 181 ccagtctgac gcccgaggag gagcgcttcc tggactcggc tgagtatggc aacatcccgg 241 tggtccggaa aatgctggag gagtccaaga cccttaactt caactgtgtg gactacatgg 301 ggcagaacgc tctgcagctg gccgtgggca acgagcacct agaggtcacg gagctgctgc 361 tgaagaagga gaacctggca cgggtggggg acgcgctgct gctggccatc agcaagggct 421 atgtgcgcat cgtggaggcc atcctcaacc acccggcctt cgcgcagggc cagcgcctga 481 cgctcagccc gctggaacag gagctgcgcg acgacgactt ctatgcctac gacgaggacg 541 gcacgcgctt ctcccacgac atcacgccta tcatcctggc ggcgcactgc caggagtatg 601 agatcgtgca catcctgctg ctcaagggcg cccgcatcga gcggccccac gactacttct 661 gcaagtgcaa tgagtgcacc gagaaacagc ggaaagactc cttcagccac tcgcgctcgc 721 gcatgaacgc ctacaaagga ctggcgagtg ctgcctactt gtccctgtcc agcgaagacc 781 ctgtcctcac cgccctggag ctcagcaacg agttagccag actagccaac attgagactg 841 aatttaagaa cgattacagg aagttatcta tgcaatgcaa ggattttgta gtgggcgtgc 901 tggacctgtg ccgagacaca gaagaggtgg aagcaatttt aaacggtgat gtgaacttcc 961 aagtctggtc cgaccaccac cgtccaagtc tgagccggat caaactcgcc attaaatatg 1021 aagtcaagaa gttcgttgct catcctaact gtcagcagca attgcttacc atgtggtatg 1081 aaaatctctc aggcttacgt caacagtcta tcgctgtgaa attcctggct gtctttggag 1141 tctccatagg cctccctttt ctcgccatag cctattggat tgctccgtgc agcaagctag 1201 gacgaaccct gaggagccct ttcatgaagt ttgtagctca tgcagtttct tttacaatct 1261 tcttgggatt attagttgtg aatgcatctg accgatttga aggtgttaaa accctgccaa 1321 acgaaacctt cacagactac ccaaaacaaa tcttcagagt gaaaaccaca cagttctcct 1381 ggacagaaat gctcattatg aagtgggtct taggaatgat ttggtccgaa tgcaaggaaa 1441 tctgggagga ggggccacgg gagtacgtgc tgcacttgtg gaacctgcta gatttcggga 1501 tgctgtccat cttcgtggcc tccttcacag cacgcttcat ggccttcctg aaggccacgg 1561 aggcacagct gtacgtggac cagcacgtgc aggacgacac gctgcacaat gtctcgcttc 1621 cgccggaagt ggcatacttc acctacgcca gggacaagtg gtggccttca gaccctcaga 1681 tcatatcgga agggctctac gcgatagccg tcgtgctgag cttctctcgc attgcataca 1741 ttctgccagc caacgagagt tttgggcccc tgcagatctc gctagggaga actgtgaaag 1801 atatcttcaa gttcatggtc attttcatca tggtatttgt ggccttcatg attgggatgt 1861 tcaacctgta ctcttactac cgaggtgcca aatacaaccc agcgtttaca acggttgaag 1921 aaagttttaa aactttgttt tggtccatat tcggcttatc tgaagtaatc tcagtggtgc 1981 tgaaatacga ccacaaattc atcgagaaca ttggctacgt tctctacggc gtttataacg 2041 tcaccatggt ggtagtgttg ctcaacatgc taatagccat gataaacaac tcctatcagg 2101 aaattgagga ggatgcagat gtggaatgga agttcgcccg agcaaaactc tggctgtctt 2161 actttgatga aggaagaact ctacctGCTC CTTTTAATCT AGTGCCAAGT CCTAAATCAT 2221 TTTATTATCT CATAATGAGA ATCAAGATGT GCCTCATAAA ACTCTGCAAA TCTAAGGCCA 2281 AAAGCTGTGA AAATGACCTT GAAATGGGCA TGCTGAATTC CAAATTCAAG AAGACTCGCT 2341 ACCAGGCTGG CATGAGGAAT TCTGAAAATC TGACAGCAAA TAACACTTTG AGCAAGCCCA 2401 CCAGATACCA GAAAATCATG AAACGGCTCA TAAAAAGATA CGTCCTGAAA GCCCAGGTGG 2461 ACAGAGAAAA TGACGAAGTC AATGAAGGCG AGCTGAAGGA AATCAAGCAA GATATCTCCA 2521 GCCTGCGCTA TGAGCTTCTT GAGGAAAAAT CTCAAGCTAC TGGTGAGCTG GCAGACCTGA 2581 TTCAACAACT CAGCGAGAAG TTTGGAAAGA ACTTAAACAA AGACCACCTG AGGGTGAACA 2641 AGGGCAAAGA CATTTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2701 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2761 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGTTTATTAC CGTTTACCAA 2821 GGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt