Construct: ORF TRCN0000474382

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008863.1_s317c1
Derived from:
ccsbBroadEn_06139
DNA Barcode:
GTACGTTCCACACTCCACCCCTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHC2 (1912)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474382
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1912 PHC2 polyhomeotic homolog 2 NM_004427.4 99.5% 99.3% (many diffs)
2 human 1912 PHC2 polyhomeotic homolog 2 NM_001330488.1 38.7% 38.6% (many diffs)
3 human 1912 PHC2 polyhomeotic homolog 2 NM_198040.2 37.4% 37.4% (many diffs)
4 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... NM_001195083.1 91.9% 96.5% (many diffs)
5 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... XM_006503237.3 91.9% 96.5% (many diffs)
6 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... XM_006503238.1 91.9% 96.5% (many diffs)
7 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... XM_006503236.1 81.3% 85.4% (many diffs)
8 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... XM_006503234.1 36.1% 38% (many diffs)
9 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... NM_001195130.1 34.9% 36.7% (many diffs)
10 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... NM_018774.4 34.9% 36.7% (many diffs)
11 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... XM_017320320.1 34.9% 36.7% (many diffs)
12 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... XR_376334.3 20% (many diffs)
13 mouse 54383 Phc2 polyhomeotic-like 2 (Drosop... XM_006503235.1 18.9% 15.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1035
ORF length:
969
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac ctcagagaac ggaaactctg cctccagcat cgccggcact gccccccaga
121atggtgagaa taaaccacca caggccattg tgaaacccca aatcctgacg catgttatcg
181aagggtttgt gatccaggag ggggcggagc ctttcccggt gggacgctcg tccctgctgg
241tggggaatct caagaagaag tatgcacagg ggttcctgcc tgagaaactt ccacagcagg
301atcacaccac caccactgac tcggagatgg aggagcccta tctgcaagaa tccaaagagg
361agggtgcccc cctcaaactc aagtgtgagc tctgtggccg ggtggacttt gcctataagt
421tcaagcgttc caagcgcttc tgttccatgg cttgtgcaaa gaggtacaac gtgggatgca
481ccaaacgggt gggacttttc cactcagacc ggagcaagct gcagaaggca ggagctgcga
541cccacaaccg ccgtcgggCC AGCAAAGCCA GTCTGCCACC ACTTACCAAG GATACCAAGA
601AGCAGCCAAC AGGCACTGTG CCCCTTTCGG TTACTGCTGC TTTGCAGCTA ACACACAGCC
661AGGAAGACTC CAGCCGTTGC TCAGATAACT CAAGCTATGA GGAACCCTTG TCACCCATCT
721CAGCCAGCTC ATCTACTTCC CGCCGGCGAC AAGGCCAGCG GGACCTGGAG CTCCCCGACA
781TGCATATGCG GGACCTGGTG GGCATGGGAC ACCACTTCCT GCCAAGTGAG CCCACCAAGT
841GGAATGTAGA AGACGTCTAC GAATTCATCC GCTCTCTGCC AGGCTGCCAG GAGATAGAAG
901AGGAATTCCG TGCCCAGGAA ATCGACGGGC AAGCCCTGCT GCTGCTCAAG GAGGACCACC
961TGATGAGCGC CATGAACATC AAGCTGGGGC CCGCCCTGAA GATCTATGCC CGCATCAGCA
1021TGCTCAAGGA CTCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1081CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1141TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTACGTTCCA CACTCCACCC
1201CTACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)