Construct: ORF TRCN0000474391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017496.1_s317c1
- Derived from:
- ccsbBroadEn_12827
- DNA Barcode:
- TTATCCGCGACACGCCTTGTATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZC3H8 (84524)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84524 | ZC3H8 | zinc finger CCCH-type conta... | NM_032494.3 | 97.7% | 97.6% | 507T>C;873_873delAinsTAAAATAGACATAAAAAGG |
| 2 | human | 84524 | ZC3H8 | zinc finger CCCH-type conta... | XM_017005107.1 | 63.4% | 63.2% | 0_1ins306;201T>C;567_567delAinsTAAAATAGACATAAAAAGG |
| 3 | human | 84524 | ZC3H8 | zinc finger CCCH-type conta... | XM_024453174.1 | 63.4% | 63.2% | 0_1ins306;201T>C;567_567delAinsTAAAATAGACATAAAAAGG |
| 4 | human | 84524 | ZC3H8 | zinc finger CCCH-type conta... | XM_024453175.1 | 63.4% | 63.2% | 0_1ins306;201T>C;567_567delAinsTAAAATAGACATAAAAAGG |
| 5 | human | 84524 | ZC3H8 | zinc finger CCCH-type conta... | XR_001738994.1 | 34.4% | (many diffs) | |
| 6 | human | 84524 | ZC3H8 | zinc finger CCCH-type conta... | XR_001738995.2 | 33.6% | (many diffs) | |
| 7 | mouse | 57432 | Zc3h8 | zinc finger CCCH type conta... | NM_020594.2 | 80.1% | 73.9% | (many diffs) |
| 8 | mouse | 57432 | Zc3h8 | zinc finger CCCH type conta... | XM_006499947.2 | 79.3% | 73.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttttgagaat cttttctcaa aaccccccaa cccggccctc ggcaaaacgg 121 ccacggactc tgacgaaaga atcgatgatg aaatagatac agaagttgaa gaaacacaag 181 aagagaaaat taaactggag tgcgagcaaa ttcccaaaaa atttagacac tctgcaatat 241 caccaaaaag ttcgctgcat agaaaatcaa gaagtaagga ctatgatgta tatagtgata 301 atgatatctg cagtcaggaa tcagaagata attttgccaa agagcttcaa cagtacatac 361 aagccagaga aatggcaaat gctgctcaac ctGAAGAATC TACAAAGAAA GAAGGAGTAA 421 AAGATACCCC ACAGGCTGCT AAACAAAAAA ATAAAAATCT TAAAGCTGGT CACAAGAATG 481 GCAAACAGAA GAAAATGAAG CGAAAATGGC CTGGCCCTGG AAACAAAGGA TCAAATGCTT 541 TGCTGAGGAA CAGCGGCTCA CAGGAAGAGG ACGGTAAACC TAAAGAGAAG CAGCAGCATT 601 TGAGTCAGGC ATTCATCAAC CAACATACAG TGGAACGCAA GGGAAAACAA ATTTGTAAAT 661 ATTTTCTTGA AAGGAAATGT ATTAAGGGAG ACCAGTGTAA ATTTGATCAT GATGCAGAGA 721 TAGAAAAGAA AAAGGAAATG TGTAAGTTTT ATGTACAAGG ATATTGTACC AGAGGTGAAA 781 ACTGTCTGTA TTTGCATAAT GAATATCCTT GTAAGTTTTA CCATACAGGA ACAAAATGTT 841 ATCAGGGAGA ATACTGCAAG TTTTCTCATG CTCCACTGAC TCCTGAAACA CAAGAATTGT 901 TGGCTAAAGT TTTGGATACT GAAAAGAAGT CATGTAATAA AATAGACATA AAAAGGTACC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GATTATCCGC GACACGCCTT GTATCTACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt