Construct: ORF TRCN0000474393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003512.1_s317c1
- Derived from:
- ccsbBroadEn_01850
- DNA Barcode:
- GAACTTTTCCATACTTAAGAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEM1 (7979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474393
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NM_006304.1 | 100% | 100% | |
| 2 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NR_163951.1 | 6.7% | (many diffs) | |
| 3 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NR_163952.1 | 6.6% | (many diffs) | |
| 4 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NR_163953.1 | 6.6% | (many diffs) | |
| 5 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NR_163950.1 | 6.4% | (many diffs) | |
| 6 | mouse | 20422 | Sem1 | SEM1, 26S proteasome comple... | NM_009169.2 | 88.5% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 279
- ORF end:
- 489
- ORF length:
- 210
- Sequence:
-
1 GTCCAGGCAC CTCGATTAGT TCTCGAGCTT TTGGAGTACG TCGTCTTTAG GTTGGGGGGA 61 GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG GAGACTGAAG TTAGGCCAGC 121 TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT 181 TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA 241 GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT GTCAGAGAAA AAGCAGCCGG 301 TAGACTTAGG TCTGTTAGAG GAAGACGACG AGTTTGAAGA GTTCCCTGCC GAAGACTGGG 361 CTGGCTTAGA TGAAGATGAA GATGCACATG TCTGGGAGGA TAATTGGGAT GATGACAATG 421 TAGAGGATGA CTTCTCTAAT CAGTTACGAG CTGAACTAGA GAAACATGGT TATAAGATGG 481 AGACTTCATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGAACTT TTCCATACTT AAGAAATTAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt