Construct: ORF TRCN0000474403

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002375.1_s317c1
Derived from:
ccsbBroadEn_03749
DNA Barcode:
CCAAGTCGCCTCGGGGTTTTTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AGPAT3 (56894)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474403
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... NM_001037553.2 100% 100%
2 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... NM_001369878.1 100% 100%
3 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... NM_001369880.1 100% 100%
4 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... NM_020132.5 100% 100%
5 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_005261160.4 100% 100%
6 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_006724029.3 100% 100%
7 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_006724030.3 100% 100%
8 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_011529665.2 100% 100%
9 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_011529660.2 85.6% 81.1% 1_58del;124_125ins112
10 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... NM_001369881.1 83.5% 83.5% 0_1ins186
11 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_006724031.3 83.5% 83.5% 0_1ins186
12 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_017028411.1 83.5% 83.5% 0_1ins186
13 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_017028412.1 83.5% 83.5% 0_1ins186
14 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_011529662.2 81.2% 81.2% 1_261del
15 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_011529664.2 81.1% 76.9% 1_124del;190_191ins112
16 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_017028408.1 76.3% 76.4% 1_202del;268_269ins112
17 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_011529663.2 74.7% 70.8% 1_232del;298_299ins112
18 human 56894 AGPAT3 1-acylglycerol-3-phosphate ... XM_011529661.2 69.4% 65.9% 1_334del;400_401ins112
19 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... NM_053014.3 86.2% 92.2% (many diffs)
20 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... XM_006513716.2 86.2% 92.2% (many diffs)
21 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... XM_006513717.3 86.2% 92.2% (many diffs)
22 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... XM_006513718.1 86.2% 92.2% (many diffs)
23 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... XM_006513719.2 86.2% 92.2% (many diffs)
24 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... XM_006513720.1 86.2% 92.2% (many diffs)
25 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... XM_006513721.1 86.2% 92.2% (many diffs)
26 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... XM_006513722.3 86.2% 92.2% (many diffs)
27 mouse 28169 Agpat3 1-acylglycerol-3-phosphate ... XM_006513723.3 86.2% 92.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1194
ORF length:
1128
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg cctgctggcc ttcctgaaga cccagttcgt gctgcacctg ctggtcggct
121ttgtcttcgt ggtgagtggt ctggtcatca acttcgtcca gctgtgcacg ctggcgctct
181ggccggtcag caagcagctc taccgccgcc tcaactgccg cctcgcctac tcactctgga
241gccaactggt catgctgctg gagtggtggt cctgcacgga gtgtacactg ttcacggacc
301aggccacggt agagcgcttt gggaaggagc acgcagtcat catcctcaac cacaacttcg
361agatcgactt cctctgtggg tggaccatgt gtgagcgctt cggagtgctg gggagctcca
421aggtcctcgc taagaaggag ctgctctacg tgcccctcat cggctggacg tggtactttc
481tggagattgt gttctgcaag cggaagtggg aggaggaccg ggacaccgtg gtcgaagggc
541tgaggcgcct gtcggactac cccgagtaca tgtggtttct cctgtactgc gaggggacgc
601gcttcacgga gaccaagcac cgcgttagca tggaggtggc ggctgctaag gggcttcctg
661tcctcaagta ccacctgctg ccgcggacca agggcttcac caccgcagtc aagtgcctcc
721gggggacagt cgcagctgtc tatgatgtaa ccctgaactt cagaggaaac aagaacccgt
781ccctgctggg gatcctctac gggaagaagt acgaggcgga catgtgcgtg aggagatttc
841ctctggaaga catCCCGCTG GATGAAAAGG AAGCAGCTCA GTGGCTTCAT AAACTGTACC
901AGGAGAAGGA CGCGCTCCAG GAGATATATA ATCAGAAGGG CATGTTTCCA GGGGAGCAGT
961TTAAGCCTGC CCGGAGGCCG TGGACCCTCC TGAACTTCCT GTCCTGGGCC ACCATTCTCC
1021TGTCTCCCCT CTTCAGTTTT GTCTTGGGCG TCTTTGCCAG CGGATCACCT CTCCTGATCC
1081TGACTTTCTT GGGGTTTGTG GGAGCAGCTT CCTTTGGAGT TCGCAGACTG ATAGGAGTAA
1141CTGAGATAGA AAAAGGCTCC AGCTACGGAA ACCAAGAGTT TAAGAAAAAG GAATACCCAA
1201CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1261ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1321TATCTTGTGG AAAGGACGAC CAAGTCGCCT CGGGGTTTTT TTCACGCGTT AAGTCgacaa
1381tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)