Transcript: Human XM_011529661.2

PREDICTED: Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3 (AGPAT3), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AGPAT3 (56894)
Length:
6155
CDS:
55..1407

Additional Resources:

NCBI RefSeq record:
XM_011529661.2
NBCI Gene record:
AGPAT3 (56894)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011529661.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419813 GACATGTGCGTGAGGAGATTT pLKO_005 1030 CDS 100% 13.200 18.480 N AGPAT3 n/a
2 TRCN0000035138 GATTGTGTTCTGCAAGCGGAA pLKO.1 696 CDS 100% 2.160 3.024 N AGPAT3 n/a
3 TRCN0000035137 GCGCTCCAGGAGATATATAAT pLKO.1 1123 CDS 100% 15.000 12.000 N AGPAT3 n/a
4 TRCN0000427286 TCGCAGACTGATAGGAGTAAC pLKO_005 1332 CDS 100% 10.800 8.640 N AGPAT3 n/a
5 TRCN0000035134 GCTACGGAAACCAAGAGTTTA pLKO.1 1373 CDS 100% 13.200 9.240 N AGPAT3 n/a
6 TRCN0000432513 CAGTTATCTTGGGAACTTAAC pLKO_005 1752 3UTR 100% 10.800 7.560 N AGPAT3 n/a
7 TRCN0000035136 CCTGAACTTCAGAGGAAACAA pLKO.1 963 CDS 100% 5.625 3.938 N AGPAT3 n/a
8 TRCN0000035135 CCTCAACCACAACTTCGAGAT pLKO.1 555 CDS 100% 4.050 2.835 N AGPAT3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011529661.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03749 pDONR223 100% 69.4% 65.9% None 1_334del;400_401ins112 n/a
2 ccsbBroad304_03749 pLX_304 0% 69.4% 65.9% V5 1_334del;400_401ins112 n/a
3 TRCN0000474403 CCAAGTCGCCTCGGGGTTTTTTTC pLX_317 30.5% 69.4% 65.9% V5 1_334del;400_401ins112 n/a
4 ccsbBroadEn_08654 pDONR223 100% 69.4% 65.9% None 1_334del;400_401ins112;606C>T n/a
5 ccsbBroad304_08654 pLX_304 0% 69.4% 65.9% V5 1_334del;400_401ins112;606C>T n/a
6 TRCN0000467671 GGAGATGCTCACATGTAGACGTCT pLX_317 36.1% 69.4% 65.9% V5 1_334del;400_401ins112;606C>T n/a
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