Construct: ORF TRCN0000474410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017118.1_s317c1
- Derived from:
- ccsbBroadEn_11216
- DNA Barcode:
- ACAGGATACCATCCAACCTGATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAV1 (7409)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474410
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7409 | VAV1 | vav guanine nucleotide exch... | NM_005428.4 | 93.1% | 91.7% | (many diffs) |
| 2 | human | 7409 | VAV1 | vav guanine nucleotide exch... | NM_001258206.2 | 90.5% | 89.1% | (many diffs) |
| 3 | human | 7409 | VAV1 | vav guanine nucleotide exch... | NM_001258207.2 | 89.3% | 87.9% | (many diffs) |
| 4 | human | 7409 | VAV1 | vav guanine nucleotide exch... | XM_005259642.1 | 87% | 80% | (many diffs) |
| 5 | mouse | 22324 | Vav1 | vav 1 oncogene | NM_001163815.1 | 82.2% | 87.6% | (many diffs) |
| 6 | mouse | 22324 | Vav1 | vav 1 oncogene | NM_011691.4 | 80% | 85.1% | (many diffs) |
| 7 | mouse | 22324 | Vav1 | vav 1 oncogene | NM_001163816.1 | 76.3% | 81% | (many diffs) |
| 8 | mouse | 22324 | Vav1 | vav 1 oncogene | XR_385313.2 | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2436
- ORF length:
- 2370
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgtgctacca ctgtactcca gactggacaa aagattcctg tgccttaaga 121 acattagaac cttcctgtcc acctgctgtg agaagttcgg cctcaagcgg agcgagctct 181 tcgaagcctt tgacctcttc gatgtgcagg attttggcaa ggtcatctac accctgtctg 241 ctctgtcctg gaccccgatc gcccagaaca gggggatcat gcccttcccc accgaggagg 301 agagtgtagg tgatgaagac atctacagtg gcctgtccga ccagatcgac gacacggtgg 361 aggaggatga ggacctgtat gactgcgtgg agaatgagga ggcggaaggc gacgagatct 421 atgaggacct catgcgctcg gagcccgtgt ccatgccgcc caagatgaca gagtatgaca 481 agcgctgctg ctgcctgcgg gagatccagc agacggagga gaagtacact gacacgctgg 541 gctccatcca gcagcatttc ttgaagcccc tgcaacggtt cctgaaacct caagacattg 601 agatcatctt tatcaacatt gaggacctgc ttcgtgttca tactcacttc ctaaaggaga 661 tgaaggaagc cctgggcacc cctggcgcag ccaatctcta ccaggtcttc atcaaataca 721 aggagaggtt cctcgtctat ggccgctact gcagccaggt ggagtcagcc agcaaacacc 781 tggaccgtgt ggccgcagcc cgggaggacg tgcagatgaa gctggaggaa tgttctcaga 841 gagccaacaa cgggaggttc accctgcggg acctgctgat ggtgcctatg cagcgagttc 901 tcaaatatca cctccttctc caggagctgg tgaaacacac gcaggaggcg atggagaagg 961 agaacctgcg gctggccctg gatgccatga gggacctggc tcagtgcgtg aacgaggtca 1021 agcgagacaa cgagacactg cgacagatca ccaatttcca gctgtccatt gagaacctgg 1081 accagtctct ggctcactat ggccggccca agatcgacgg ggaactcaag atcacctcgg 1141 tggaacggcg ctccaagatg gacaggtatg ccttcctgct cgacaaagct ctactcatct 1201 gtaagcgcag gggagactcc tatgacctca aggactttgt aaacctgcac agcttccagg 1261 ttcgggatga ctcttcagga gaccgagaca acaagaagtg gagccacatg ttcctcctga 1321 tcgaggacca aggtgcccag ggctatgagc tgttcttcaa gacaagagaa ttgaagaaga 1381 agtggatgga gcagtttgag atggccatct ccaacatcta tccggagaat gccaccgcca 1441 acgggcatga cttccagatg ttctcctttg aggagaccac atcctgcaag gcctgtcaga 1501 tgctgcttag aggtaccttc tatcagggct accgctgcca tcggtgccgg gcatctgcac 1561 acaaggagtg tctggggagg gtccctccat gtggccgaca tgggcaagat ttcccaggaa 1621 ctatgaagaa ggacaaacta catcgcaggg ctcaggacaa aaagaggaat gagctgggtc 1681 tgcccaagat ggaggtgttt caggaatact acgggcttcc tccaccccct ggagccattg 1741 gaccctttct acggctcaac cctggagaca ttgtggagct cacgaaggct gaggctgaac 1801 agaactggtg ggagggcaga aatacatcta ctaatgaaat tggctggttt ccttgtaaca 1861 gggtgaagcc ctatgtccat ggccctcctc aggacctgtc tgttcatctc tggtacgcag 1921 gccccatgga gcgggcaggg gcagagagca tcctggccaa CCGCTCGGAC GGGACTTTCT 1981 TGGTGCGGCA GAGGGTGAAG GATGCAGCAG AATTTGCCAT CAGCATTAAA TATAACGTCG 2041 AGGTCAAGCA CATTAAAATC ATGACAGCAG AAGGACTGTA CCGGATCACA GAGAAAAAGG 2101 CTTTCCGGGG GCTTACGGAG CTGGTGGAGT TTTACCAGCA GAACTCTCTA AAGGATTGCT 2161 TCAAGTCTCT GGACACCACC TTGCAGTTCC CCTTCAAGGA GCCTGAAAAG AGAACCATCA 2221 GCAGGCCAGC AGTGGGAAGC ACAAAGTATT TTGGCACAGC CAAAGCCCGC TATGACTTCT 2281 GCGCCCGAGA CCGATCAGAG CTGTCGCTCA AGGAGGGTGA CATCATCAAG ATCCTTAACA 2341 AGAAGGGACA GCAAGGCTGG TGGCGAGGGG AGATCTATGG CCGGGTTGGC TGGTTCCCTG 2401 CCAACTACGT GGAGGAAGAT TATTCTGAAT ACTGCTGCCC AACTTTCTTG TACAAAGTGG 2461 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2521 CTAGTCCGTA ACTTGAAAGA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 2581 ACAGGATACC ATCCAACCTG ATTTACGCGT TAAGTCgaca atcaacctct ggattacaaa 2641 atttgtgaaa gatt