Construct: ORF TRCN0000474419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013098.1_s317c1
- Derived from:
- ccsbBroadEn_13878
- DNA Barcode:
- GCCTTGGGTCGAAGTCGCCCGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDST1 (3340)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474419
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3340 | NDST1 | N-deacetylase and N-sulfotr... | NM_001301063.2 | 66% | 63.7% | (many diffs) |
| 2 | human | 3340 | NDST1 | N-deacetylase and N-sulfotr... | NM_001543.5 | 61.7% | 59.6% | (many diffs) |
| 3 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | NM_001348146.1 | 79% | 84% | (many diffs) |
| 4 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | XM_011246846.2 | 71.5% | 71.3% | (many diffs) |
| 5 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | NM_001348100.1 | 56.1% | 58.5% | (many diffs) |
| 6 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | NM_001348101.1 | 56.1% | 58.5% | (many diffs) |
| 7 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | NM_001348102.1 | 56.1% | 58.5% | (many diffs) |
| 8 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | NM_001348103.1 | 56.1% | 58.5% | (many diffs) |
| 9 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | NM_008306.5 | 56.1% | 58.5% | (many diffs) |
| 10 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | XM_006525667.3 | 56.1% | 58.5% | (many diffs) |
| 11 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | XM_006525668.2 | 56.1% | 58.5% | (many diffs) |
| 12 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | XM_006525669.3 | 56.1% | 58.5% | (many diffs) |
| 13 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | XM_006525670.3 | 56.1% | 58.5% | (many diffs) |
| 14 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | XM_006525671.1 | 56.1% | 58.5% | (many diffs) |
| 15 | mouse | 15531 | Ndst1 | N-deacetylase/N-sulfotransf... | XM_011246844.2 | 56.1% | 58.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1734
- ORF length:
- 1668
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgccctggca tgcctccgga ggctgtgtcg gcacgtgtcc ccgcaggctg 121 tccttttcct gctgttagtc ttctgcctgt tcagcgtttt catctcggcc tactacctat 181 atggctggaa gcgaggcctg gagccctcgg cggatgcccc cgagcctgac tgcggggacc 241 cgccgcctgt ggcccccagt cgcctgctgc cactcaagcc tgtgcaggca gccacccctt 301 cccgcacaga cccgttggtg ctggtctttg tggagagcct ctactcgcaa ctgggccagg 361 aggtggtggc catcctggag tccagccgct tcaaataccg cacagagatt gcgccgggca 421 agggtgacat gcccacgctc actgacaagg gccgtggccg cttcgccctc atcatctatg 481 agaacatcct caagtatgtc aacctggacg cctggaaccg ggagctgctg gacaagtact 541 gtgtggccta cggcgtgggc atcattggct tcttcaaggc caatgagaac agcctgctga 601 gtgcgcagct caagggcttc cccctgttcc tgcactcaaa cctgggcctg aaggactgca 661 gcatcaaccc caagtccccg ctgctctacg tgacgcgacc tagcgaggtg gagaaaggtg 721 tgctccccgg cgaggactgg acggttttcc agtcaaatca ctccacctat gagccagtgc 781 tgctggccaa gacgcgctcg tctgagtcca tcccacacct gggcgcagac gccggcctgc 841 atgctgcact gcacgccact gtggtccagg acctgggcct gcacgacggc atccagcgcg 901 tgctgtttgg caacaacctg aacttctggc tgcacaagct tgtcttcgtg gatgccgtgg 961 ccttcctcac ggggaagcgc ctctccctgc cattggaccg ctacatcctg gtggacattg 1021 atgacatctt cgtgggcaag gagggcacac gcatgaaggt ggaggacgtg aaggccctgt 1081 ttgacacaca gaacgaacta cgcgcacaca tcccaaactt caccttcaac ctgggctact 1141 cagggaaatt cttccagaca ggtaccaatg ctgaggacgc tggggatgat ctgctgctgt 1201 cgtatgtgaa ggagttctgg tggttccccc acatgtGGAG CCACATGCAG CCCCACCTTT 1261 TCCACAACCA GTCCGTGTTG GCCGAGCAGA TGGCCTTGAA CAAGAAGTTC GCTGTCGAGC 1321 ATGGCATTCC CACAGACATG GGGTATGCAG TGGCGCCCCA CCACTCGGGC GTGTACCCCG 1381 TGCACGTGCA GCTGTACGAG GCTTGGAAGC AGGTGTGGAG CATCCGCGTG ACCAGCACGG 1441 AGGAGTACCC CCACCTGAAG CCAGCCCGCT ACCGCCGTGG CTTCATCCAC AATGGCATCA 1501 TGGTTCTCCC ACGGCAGACC TGCGGCCTCT TCACACACAC CATCTTCTAC AACGAGTACC 1561 CTGGCGGCTC CAGTGAGCTG GACAAGATCA TCAACGGGGG CGAGCTCTTC CTCACCGTGC 1621 TCCTCAATCC TGTGAGTGCT CCACAGCCCA TGGCTGCTGG TGAGAAGGGG CTGCTGCACA 1681 GCCTGTCTGC AGCTGACACA GGCTTCCTGG AGCCAGGAAA GGGTGGAGAG GCATGCCCAA 1741 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1801 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1861 TATCTTGTGG AAAGGACGAG CCTTGGGTCG AAGTCGCCCG TTAACGCGTT AAGTCgacaa 1921 tcaacctctg gattacaaaa tttgtgaaag att