Construct: ORF TRCN0000474420
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001540.1_s317c1
- Derived from:
- ccsbBroadEn_12467
- DNA Barcode:
- TTGCTGAGTACCGGGTCAGTGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS1BP3 (64342)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474420
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004701.1 | 65.8% | 66.4% | 546C>T;623_624insGTTGGT;628_939delinsTGTTGT |
| 2 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004698.1 | 53.2% | 53.6% | 546C>T;623_624insGTTGGT;628_1164delinsTGTTGT |
| 3 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | NM_022460.4 | 52.6% | 53% | 546C>T;623_624insGTTGGT;628_1176delinsTGTTGT |
| 4 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004697.2 | 52.2% | 52.6% | 546C>T;623_624insGTTGGT;628_1185delinsTGTTGT |
| 5 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004696.2 | 52.1% | 52.5% | 546C>T;623_624insGTTGGT;628_1188delinsTGTTGT |
| 6 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004700.1 | 44.3% | 44.6% | (many diffs) |
| 7 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004699.2 | 43.9% | 44.1% | (many diffs) |
| 8 | mouse | 58240 | Hs1bp3 | HCLS1 binding protein 3 | NM_021429.3 | 43.8% | 42.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gtccccggcg gtgctcgtca cctccaggcg acttcagaat gcccacactg 121 gcctcgacct gactgtgccc cagcaccagg aggtacgggg caagatgatg tctGGACACG 181 TGGAGTACCA GATCCTGGTG GTGACCCGTC TGGCTGCGTT CAAGTCGGCC AAGCACAGGC 241 CCGAGGATGT CGTCCAGTTC TTGGTCTCCA AAAAGTACAG CGAGATTGAG GAGTTTTACC 301 AGAAACTGAG CAGTCGTTAT GCAGCAGCCA GCCTCCCCCC ACTACCCAGG AAGGTCCTGT 361 TTGTTGGGGA GTCTGACATC CGGGAGAGGA GAGCCGTGTT CAATGAGATC CTGCGCTGTG 421 TCTCCAAGGA TGCCGAGTTG GCAGGCAGCC CAGAGCTGCT AGAGTTCTTA GGTACCAGAT 481 CCCCAGGGGC TGCAGGGCTC ACCAGCAGAG ATTCCTCTGT CCTGGATGGC ACAGACAGTC 541 AGACAGGGAA TGATGAAGAG GCTTTCGACT TTTTTGAGGA GCAAGACCAA GTGGCAGAAG 601 AGGGTCCGCC TGTCCAGAGC CTGAAGGGCG AGGATGCTGA GGAATCCTTG GAGGAGGAGG 661 AGGCGCTGGA CCCTCTGGGC ATTATGCGGT TGGTCTCCTG TTGTTGCCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA TTGCTGAGTA CCGGGTCAGT GAATACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt