Construct: ORF TRCN0000474420

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001540.1_s317c1
Derived from:
ccsbBroadEn_12467
DNA Barcode:
TTGCTGAGTACCGGGTCAGTGAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HS1BP3 (64342)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474420
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64342 HS1BP3 HCLS1 binding protein 3 XM_017004701.1 65.8% 66.4% 546C>T;623_624insGTTGGT;628_939delinsTGTTGT
2 human 64342 HS1BP3 HCLS1 binding protein 3 XM_017004698.1 53.2% 53.6% 546C>T;623_624insGTTGGT;628_1164delinsTGTTGT
3 human 64342 HS1BP3 HCLS1 binding protein 3 NM_022460.4 52.6% 53% 546C>T;623_624insGTTGGT;628_1176delinsTGTTGT
4 human 64342 HS1BP3 HCLS1 binding protein 3 XM_017004697.2 52.2% 52.6% 546C>T;623_624insGTTGGT;628_1185delinsTGTTGT
5 human 64342 HS1BP3 HCLS1 binding protein 3 XM_017004696.2 52.1% 52.5% 546C>T;623_624insGTTGGT;628_1188delinsTGTTGT
6 human 64342 HS1BP3 HCLS1 binding protein 3 XM_017004700.1 44.3% 44.6% (many diffs)
7 human 64342 HS1BP3 HCLS1 binding protein 3 XM_017004699.2 43.9% 44.1% (many diffs)
8 mouse 58240 Hs1bp3 HCLS1 binding protein 3 NM_021429.3 43.8% 42.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
705
ORF length:
639
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gtccccggcg gtgctcgtca cctccaggcg acttcagaat gcccacactg
121gcctcgacct gactgtgccc cagcaccagg aggtacgggg caagatgatg tctGGACACG
181TGGAGTACCA GATCCTGGTG GTGACCCGTC TGGCTGCGTT CAAGTCGGCC AAGCACAGGC
241CCGAGGATGT CGTCCAGTTC TTGGTCTCCA AAAAGTACAG CGAGATTGAG GAGTTTTACC
301AGAAACTGAG CAGTCGTTAT GCAGCAGCCA GCCTCCCCCC ACTACCCAGG AAGGTCCTGT
361TTGTTGGGGA GTCTGACATC CGGGAGAGGA GAGCCGTGTT CAATGAGATC CTGCGCTGTG
421TCTCCAAGGA TGCCGAGTTG GCAGGCAGCC CAGAGCTGCT AGAGTTCTTA GGTACCAGAT
481CCCCAGGGGC TGCAGGGCTC ACCAGCAGAG ATTCCTCTGT CCTGGATGGC ACAGACAGTC
541AGACAGGGAA TGATGAAGAG GCTTTCGACT TTTTTGAGGA GCAAGACCAA GTGGCAGAAG
601AGGGTCCGCC TGTCCAGAGC CTGAAGGGCG AGGATGCTGA GGAATCCTTG GAGGAGGAGG
661AGGCGCTGGA CCCTCTGGGC ATTATGCGGT TGGTCTCCTG TTGTTGCCCA ACTTTCTTGT
721ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
781AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
841GAAAGGACGA TTGCTGAGTA CCGGGTCAGT GAATACGCGT TAAGTCgaca atcaacctct
901ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)