Construct: ORF TRCN0000474422
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000254.1_s317c1
- Derived from:
- ccsbBroadEn_13503
- DNA Barcode:
- CGTCATTATATTCCTCTCCAGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP170B (283638)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474422
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283638 | CEP170B | centrosomal protein 170B | XM_011536667.2 | 15.2% | 15.2% | 1_2568del |
| 2 | human | 283638 | CEP170B | centrosomal protein 170B | NM_015005.3 | 10.1% | 10.1% | 1_4095del |
| 3 | human | 283638 | CEP170B | centrosomal protein 170B | NM_001112726.3 | 9.9% | 9.9% | 1_4200del |
| 4 | human | 283638 | CEP170B | centrosomal protein 170B | XM_005267550.4 | 9.6% | 9.6% | 1_4305del |
| 5 | human | 283638 | CEP170B | centrosomal protein 170B | XM_011536665.2 | 9.6% | 9.6% | 1_4305del |
| 6 | human | 283638 | CEP170B | centrosomal protein 170B | XM_011536666.2 | 9.6% | 9.6% | 1_4305del |
| 7 | human | 283638 | CEP170B | centrosomal protein 170B | XM_017021228.1 | 9.4% | 9.4% | 1_4428del |
| 8 | human | 283638 | CEP170B | centrosomal protein 170B | XM_017021227.1 | 9.2% | 9.2% | 1_4533del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 tcttCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCT GAACCCGGTG TCCCAGCTGT CGCAGGCCAT CCGTGAGAAC ACAGAGCACC 121 TTGCCGAGAA GATGAAGATC CTCTTTCAGA ACACAGGGAG AGCTTGGGAG GACCTGGAAG 181 CCAGGATCAA CGCCGAGAAC GAGGTGCCCA TCCTGAAGAC ATCTAACAAG GAAATCAGCT 241 CCATCCTGAA GGAACTGAGG CGGGTGCAGA AACAGCTGGA AGTTATCAAT GCCATCGTGG 301 ACCCCAGTGG GAGCCTGGAC CTGCTCACAG GAAACAGGAG CTTGGCCAGC TCTGCACAGC 361 CGGGGCTGGG GAAGGGCCGC GTGGCTGCCC AGAGCCCACC CTCACCCGCC TCAGCCGAGG 421 CCCTGCTGCC AGCCCTGCCC CTGAGGAATT TCCCACAGCG GGCCAGCTGT GGGCCTCCCA 481 GCCTCCCGGA CCCCACCTTC CTCCCTGATG CCGAGAGGTT CCTGATCTAC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGACGTCATT ATATTCCTCT CCAGGATACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt