Construct: ORF TRCN0000474428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007319.1_s317c1
- Derived from:
- ccsbBroadEn_12284
- DNA Barcode:
- GGGCGCGAGGCGGTCAGCTCTGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM165 (55858)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474428
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55858 | TMEM165 | transmembrane protein 165 | XM_017008412.1 | 78.5% | 78.5% | 1_168del |
| 2 | human | 55858 | TMEM165 | transmembrane protein 165 | NM_018475.5 | 63.2% | 63.2% | 1_357del |
| 3 | human | 55858 | TMEM165 | transmembrane protein 165 | XM_011534394.3 | 58% | 55.7% | (many diffs) |
| 4 | human | 55858 | TMEM165 | transmembrane protein 165 | NR_073070.2 | 30.2% | 1_693del;1309_2034del | |
| 5 | human | 55858 | TMEM165 | transmembrane protein 165 | XR_001741287.2 | 12.1% | 1_894del;1328_1506del;1689_5058del | |
| 6 | mouse | 21982 | Tmem165 | transmembrane protein 165 | NM_011626.2 | 56.1% | 61.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aatgcgctat aaccgcctga ccgtgctggc tggtgcaatg cttgccttGG 121 GACTAATGAC ATGCTTGTCA GTTTTGTTTG GCTATGCCAC CACAGTCATC CCCAGGGTCT 181 ATACATACTA TGTTTCAACT GTATTATTTG CCATTTTTGG CATTAGAATG CTTCGGGAAG 241 GCTTAAAGAT GAGCCCTGAT GAGGGTCAAG AGGAACTGGA AGAAGTTCAA GCTGAATTAA 301 AGAAGAAAGA TGAAGAATTT CAACGAACCA AACTTTTAAA TGGACCGGGA GATGTTGAAA 361 CGGGTACAAG CATAACAGTA CCTCAGAAAA AGTGGTTGCA TTTTATTTCA CCCATTTTTG 421 TTCAAGCTCT TACATTAACA TTCTTAGCAG AATGGGGTGA TCGCTCTCAA CTAACTACAA 481 TTGTATTGGC AGCTAGAGAG GACCCCTATG GTGTAGCCGT GGGTGGAACT GTGGGGCACT 541 GCCTGTGCAC GGGATTGGCA GTAATTGGAG GAAGAATGAT AGCACAGAAA ATCTCTGTCA 601 GAACTGTGAC AATCATAGGA GGCATCGTTT TTTTGGCGTT TGCATTTTCT GCACTATTTA 661 TAAGCCCTGA TTCTGGTTTT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGGC GCGAGGCGGT 841 CAGCTCTGCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt