Transcript: Human NM_018475.5

Homo sapiens transmembrane protein 165 (TMEM165), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
TMEM165 (55858)
Length:
1931
CDS:
234..1208

Additional Resources:

NCBI RefSeq record:
NM_018475.5
NBCI Gene record:
TMEM165 (55858)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_018475.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000157390 GCTTCGGGAAGGCTTAAAGAT pLKO.1 755 CDS 100% 5.625 3.938 N TMEM165 n/a
2 TRCN0000153034 GAGATGTTGAAACGGGTACAA pLKO.1 874 CDS 100% 4.950 3.465 N TMEM165 n/a
3 TRCN0000153771 CTGACTTCTAAGTGTGGGTTT pLKO.1 1397 3UTR 100% 4.050 2.835 N TMEM165 n/a
4 TRCN0000153606 CTTGGGACTAATGACATGCTT pLKO.1 641 CDS 100% 3.000 2.100 N TMEM165 n/a
5 TRCN0000156701 CCTGACTTCTAAGTGTGGGTT pLKO.1 1396 3UTR 100% 2.640 1.848 N TMEM165 n/a
6 TRCN0000158098 CCTTGGGACTAATGACATGCT pLKO.1 640 CDS 100% 2.640 1.848 N TMEM165 n/a
7 TRCN0000152975 GATTGGCAGTAATTGGAGGAA pLKO.1 1078 CDS 100% 2.640 1.848 N TMEM165 n/a
8 TRCN0000151349 GAGCCAGTAAACAGTAGTTTA pLKO.1 1637 3UTR 100% 1.320 0.924 N TMEM165 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_018475.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12284 pDONR223 100% 63.2% 63.2% None 1_357del n/a
2 ccsbBroad304_12284 pLX_304 0% 63.2% 63.2% V5 1_357del n/a
3 TRCN0000474428 GGGCGCGAGGCGGTCAGCTCTGCT pLX_317 90.2% 63.2% 63.2% V5 1_357del n/a
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