Construct: ORF TRCN0000474430
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010717.1_s317c1
- Derived from:
- ccsbBroadEn_06757
- DNA Barcode:
- GAGCCTGAAAGAGTTCCCCAGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIB (5479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474430
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5479 | PPIB | peptidylprolyl isomerase B | NM_000942.4 | 99.8% | 99.5% | 633T>A |
| 2 | mouse | 19035 | Ppib | peptidylprolyl isomerase B | NM_011149.2 | 90.1% | 93.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcgcctctcc gaacgcaaca tgaaggtgct ccttgccgcc gccctcatcg 121 cggggtccgt cttcttcctg ctgctgccgg gaccttctgc ggccgatgag aagaagaagg 181 ggcccaaagt caccgtcaag gtgtattttg acctacgaat tggagatgaa gatgtaggcc 241 gggtgatctt tggtctcttc ggaaagactg ttccaaaaac agtggataat tttgtggcct 301 tagctacagg agagaaagga tttGGCTACA AAAACAGCAA ATTCCATCGT GTAATCAAGG 361 ACTTCATGAT CCAGGGCGGA GACTTCACCA GGGGAGATGG CACAGGAGGA AAGAGCATCT 421 ACGGTGAGCG CTTCCCCGAT GAGAACTTCA AACTGAAGCA CTACGGGCCT GGCTGGGTGA 481 GCATGGCCAA CGCAGGCAAA GACACCAACG GCTCCCAGTT CTTCATCACG ACAGTCAAGA 541 CAGCCTGGCT AGATGGCAAG CATGTGGTGT TTGGCAAAGT TCTAGAGGGC ATGGAGGTGG 601 TGCGGAAGGT GGAGAGCACC AAGACAGACA GCCGGGATAA ACCCCTGAAG GATGTGATCA 661 TCGCAGACTG CGGCAAGATC GAGGTGGAGA AGCCCTTAGC CATCGCCAAG GAGTACCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAG AGCCTGAAAG AGTTCCCCAG TGAACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att