Construct: ORF TRCN0000474433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009769.1_s317c1
- Derived from:
- ccsbBroadEn_12788
- DNA Barcode:
- TCCAATTATTAGAGTGCACCGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOXL4 (84171)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84171 | LOXL4 | lysyl oxidase like 4 | NM_032211.7 | 35% | 35% | 1_1473del |
| 2 | human | 84171 | LOXL4 | lysyl oxidase like 4 | XM_005270216.2 | 35% | 35% | 1_1476del |
| 3 | human | 84171 | LOXL4 | lysyl oxidase like 4 | XM_006718020.1 | 35% | 35% | 1_1476del |
| 4 | human | 84171 | LOXL4 | lysyl oxidase like 4 | XM_024448232.1 | 35% | 35% | 1_1476del |
| 5 | mouse | 67573 | Loxl4 | lysyl oxidase-like 4 | NM_053083.3 | 30.5% | 31.8% | (many diffs) |
| 6 | mouse | 67573 | Loxl4 | lysyl oxidase-like 4 | NM_001164311.1 | 30.5% | 31.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 861
- ORF length:
- 795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tggggtgcgc tgctcaggca cagagctggc cctgcagcag tgccagaggc 121 acgggccggt gcactgctcc cacggtggcg ggcgcttcct ggctggagtc tcctgcatgg 181 acagtgcacc agacctggtg atgaacgccc agctagtgca ggagacggcc tacttggagg 241 accgcccgct cagccagctg tattgtgccc acgaggagaa ctgcctctcc aagtctgcgg 301 atcacatgga ctggccctac ggataccgcc gcctattgcg cttctccaca cagatctaca 361 atctgggccg gactgacttt cgtccaaaga ctggacgcga tagctgggtt tggcaccagt 421 gccacaggca ttaccacagc attgaggtct tcacccacta cgaccTCCTC ACTCTCAATG 481 GCTCCAAGGT GGCTGAGGGG CACAAGGCCA GCTTCTGTCT GGAGGACACA AACTGCCCCA 541 CAGGACTGCA GCGGCGCTAC GCATGTGCCA ACTTTGGAGA ACAGGGAGTG ACTGTAGGCT 601 GCTGGGACAC CTACCGGCAT GACATTGATT GCCAGTGGGT GGATATCACA GATGTGGGCC 661 CCGGGAATTA TATCTTCCAG GTGATTGTGA ACCCCCACTA TGAAGTGGCA GAGTCAGATT 721 TCTCCAACAA TATGCTGCAG TGCCGCTGCA AGTATGATGG GCACCGGGTC TGGCTGCACA 781 ACTGCCACAC AGGGAATTCA TACCCAGCCA ATGCAGAACT CTCCCTGGAG CAGGAACAGC 841 GTCTCAGGAA CAACCTCATC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 901 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 961 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCCA ATTATTAGAG 1021 TGCACCGCAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt