Construct: ORF TRCN0000474437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002033.1_s317c1
- Derived from:
- ccsbBroadEn_15753
- DNA Barcode:
- CTCTTTTGGGTTCCCTAAAGGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASTN2 (23245)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474437
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23245 | ASTN2 | astrotactin 2 | NM_001184735.1 | 99.6% | 98.9% | 174A>T;176T>A |
| 2 | human | 23245 | ASTN2 | astrotactin 2 | NM_001184734.1 | 49.5% | 46% | (many diffs) |
| 3 | human | 23245 | ASTN2 | astrotactin 2 | NM_198188.2 | 47% | 43.6% | (many diffs) |
| 4 | human | 23245 | ASTN2 | astrotactin 2 | NM_198187.3 | 46.2% | 42.9% | (many diffs) |
| 5 | human | 23245 | ASTN2 | astrotactin 2 | NM_198186.3 | 42.2% | 39.2% | (many diffs) |
| 6 | human | 23245 | ASTN2 | astrotactin 2 | NM_014010.5 | 14.4% | 13.4% | (many diffs) |
| 7 | human | 23245 | ASTN2 | astrotactin 2 | NM_001365069.1 | 13.9% | 12.9% | (many diffs) |
| 8 | human | 23245 | ASTN2 | astrotactin 2 | NM_001365068.1 | 13.9% | 12.9% | (many diffs) |
| 9 | mouse | 56079 | Astn2 | astrotactin 2 | NM_019514.3 | 13.2% | 13% | (many diffs) |
| 10 | mouse | 56079 | Astn2 | astrotactin 2 | XM_006538104.3 | 12.7% | 12.6% | (many diffs) |
| 11 | mouse | 56079 | Astn2 | astrotactin 2 | NM_207109.2 | 12.7% | 12.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cttcatcacc tacctctcag gtttgctgac agcccagatg ctgtcagatg 121 accagctcat ttcaggtgtg gagattcgct gtgaggagaa ggggcgctgt ccatctacct 181 gtcacctttg ccgccggcca ggcaaggagc agctgagccc cacaccagtg ctgctggata 241 acaaccgtgt ggtgccactt tatacccTCA TCCAAGACAA TGGCACAAAG GAGGCCTTCA 301 AGAGTGCACT GATGAGTTCC TACTGGTGCT CAGGGAAAGG GGATGTGATC GATGACTGGT 361 GCAGGTGTGA CCTCAGCGCC TTTGATGCCA ATGGGCTCCC CAACTGCAGC CCCCTTCTGC 421 AGCCGGTGCT GCGGCTGTCC CCAACAGTGG AGCCCTCCAG TACTGTGGTC TCCTTGGAGT 481 GGGTGGATGT TCAGCCAGCT ATTGGGACCA AGGTCTCCGA CTATATTCTG CAGCATAAGA 541 AAGTGGATGA ATACACAGAC ACTGACCTGT ACACAGTTTA TTGCTGGATT ACATTTATTG 601 ATTTGCGGAT ATTGAACCAG CCTTGCATCC CAGGGATGAA GCCAACTTGC CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGACTCTTTT GGGTTCCCTA AAGGGCGACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt