Construct: ORF TRCN0000474446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002654.1_s317c1
- Derived from:
- ccsbBroadEn_15993
- DNA Barcode:
- CCTTTAGGCTTATCGCACCGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMKMT (79823)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004977.1 | 58.1% | 58.1% | 1_285del |
| 2 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004978.1 | 58.1% | 58.1% | 1_285del |
| 3 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004979.2 | 55.8% | 55% | (many diffs) |
| 4 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004976.1 | 55.6% | 53.6% | (many diffs) |
| 5 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004975.1 | 55.6% | 54.9% | (many diffs) |
| 6 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004980.1 | 55.3% | 55% | (many diffs) |
| 7 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004981.1 | 55.2% | 55% | 1_285del;662_663delTTinsGGGAAGCAACCAGGGGAAAA |
| 8 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_011533111.2 | 54.9% | 54.3% | (many diffs) |
| 9 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004972.1 | 52.9% | 51.4% | (many diffs) |
| 10 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004973.2 | 52.9% | 51.4% | (many diffs) |
| 11 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004974.2 | 52.9% | 51.4% | (many diffs) |
| 12 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004971.1 | 52% | 50.6% | (many diffs) |
| 13 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XM_017004970.1 | 50.6% | 49.8% | (many diffs) |
| 14 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XR_939723.1 | 44.7% | (many diffs) | |
| 15 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XR_939722.2 | 44.7% | (many diffs) | |
| 16 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XR_001738954.1 | 43.5% | (many diffs) | |
| 17 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | NM_024766.5 | 39.5% | 39% | (many diffs) |
| 18 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XR_001738952.1 | 39% | (many diffs) | |
| 19 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XR_001738951.1 | 32.7% | (many diffs) | |
| 20 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XR_001738950.1 | 22.7% | (many diffs) | |
| 21 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XR_001738949.2 | 22.4% | (many diffs) | |
| 22 | human | 79823 | CAMKMT | calmodulin-lysine N-methylt... | XR_001738953.1 | 9.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 462
- ORF length:
- 396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatGGA GTCGCGAGTC GCGGACGCTG GGACCGGCGA GACCGCGCGA GCAGCGGGCG 121 GGAGTCCGGC AGTTGGCTGC ACCACTCGGG GGCCCGTAGT CTCGGCGCCC CTGGGAGCCG 181 CCCGGTGGAA GCTCCTGCGG CAGGTTCTGA AGCAAAAACA CCTGGATGAT TGCCTGCGAC 241 ATGTATCTGT AAGAAGATTT GAATCATTTA ATCTGTTTTC AGTAACAGAA GGCAAAGAAA 301 GGGAAACTGA AGAGGAGGTT GGTGCATGGG TCCAATATAC AAGCATCTTC TGTCCTGAAT 361 ACAGTATCTC CTTAAGGCAT AATAGTGGAT CCTTGAATGT TGAAGATGTC CTTACCAGCT 421 TTGACAATAC AGGAAATGTT TGGGAAGCAA CCAGGGGAAA ATGCCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGACCT TTAGGCTTAT CGCACCGGAA CACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t