Construct: ORF TRCN0000474447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007623.1_s317c1
- Derived from:
- ccsbBroadEn_07567
- DNA Barcode:
- TGCATATCCCCCGCTCTTACTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MPHOSPH6 (10200)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474447
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10200 | MPHOSPH6 | M-phase phosphoprotein 6 | NM_005792.2 | 99.1% | 99.3% | (many diffs) |
| 2 | human | 10200 | MPHOSPH6 | M-phase phosphoprotein 6 | XM_011522808.3 | 88.5% | 88.7% | 0_1ins54;366A>G |
| 3 | mouse | 68533 | Mphosph6 | M phase phosphoprotein 6 | NM_026758.3 | 82.8% | 87.5% | (many diffs) |
| 4 | mouse | 68533 | Mphosph6 | M phase phosphoprotein 6 | XM_011248495.2 | 73.4% | 77% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccgagaga aagacaaagt tgtccaagaa tctgctgcgc atgaagttta 121 tgcaaagggg actggactca gaaaccaaga aacaactaga agaagaagaa aagaaaaTCA 181 TTAGTGAAGA GCACTGGTAC TTGGATTTGC CAGAGCTTAA AGAGAAAGAG AGTTTCATAA 241 TAGAAGAGCA GAGTTTCTTA CTATGTGAAG ATCTTCTCTA TGGAAGAATG TCATTCAGAG 301 GATTTAATCC TGAGGTTGAG AAATTGATGC TTCAGATGAA TGCTAAGCAC AAAGCAGAAG 361 AAGTTGAAGA TGAAACAGTA GAGCTTGATG TGTCAGATGA AGAGATGGCT AGAAGATATG 421 AGACCTTGGT GGGGACAATT GGGAAAAAGT TTGCCAGAAA GAGAGACCAT GCCAATTATG 481 AAGAGGATGA AAATGGAGAC ATAACACCAA TTAAAGCAAA GAAGATGTTC TTAAAGCCCC 541 AGGATTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGCATATCC CCCGCTCTTA CTGTTACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt