Construct: ORF TRCN0000474453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005315.1_s317c1
- Derived from:
- ccsbBroadEn_07467
- DNA Barcode:
- TGCGTTCCGCAAATACCAGACGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRORP (9692)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9692 | PRORP | protein only RNase P cataly... | NM_014672.4 | 99.9% | 100% | 87C>G |
| 2 | human | 9692 | PRORP | protein only RNase P cataly... | XM_005268237.3 | 99.9% | 100% | 87C>G |
| 3 | human | 9692 | PRORP | protein only RNase P cataly... | XM_011537410.2 | 99.9% | 100% | 87C>G |
| 4 | human | 9692 | PRORP | protein only RNase P cataly... | NM_001256678.2 | 97.1% | 97.2% | 87C>G;984_985ins48 |
| 5 | human | 9692 | PRORP | protein only RNase P cataly... | XM_017021836.1 | 97.1% | 97.2% | 87C>G;984_985ins48 |
| 6 | human | 9692 | PRORP | protein only RNase P cataly... | NM_001256679.2 | 83.7% | 83.7% | 0_1ins285 |
| 7 | human | 9692 | PRORP | protein only RNase P cataly... | NM_001256680.2 | 36.1% | 36.1% | 0_1ins1116 |
| 8 | human | 9692 | PRORP | protein only RNase P cataly... | NM_001256681.1 | 36.1% | 36.1% | 0_1ins1116 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1815
- ORF length:
- 1749
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tttctatttg tttggtattc gaagctttcc gaagctttgg aagagcccat 121 accttgggct aggcccaggg cactcttatg tgtcgctgtt tctggcagac cgctgtggca 181 tcaggaacca gcagaggttg ttttctctta aaacaatgtc tccacagaat accaaagcaa 241 cgaatctgat tgccaaggcc agatatctca ggaaagatga gggcagtaat aagcaagttt 301 attctgttcc tcattttttt ttagctggag cagctaagga gagatcacag atgaattctc 361 aaactgaaga tcatgccttg gcacctgtga ggaacactat tcaactccca acacaacctt 421 tgaattcaga ggagtgggat aaacttaagg aagatttaaa agaaaacacc ggaaagacca 481 gtttcgaaag ttggatcatt tcacagatgg ctggctgtca tagctctata gatgtggcta 541 aatctctgct ggcatgggta gcagccaaaa ataatggtat tgtaagttac gatttactgg 601 tcaagtattt gtatctctgt gtctttcata tgcagacatc tgaagttatt gatgtctttg 661 aaattatgaa agccagatat aagactttag aacctagagg ttacagtctt ctcatccggg 721 gattgatcca ttcagacaga tggagagaag cattgttgct gttagaggac atcaaaaaag 781 ttataactcc ttcaaaaaag aactataatg actgtatcca gggagctctc cttcatcaag 841 atgtaaacac agcttggaat ttatatcagg aattgctagg tcatgatatt gttcctatgt 901 tggaaacttt aaaagctttc tttgattttg gaaaagacat aaaggatgat aactattcaa 961 ataaactact agatattctt tcatatctaa gaaataatca gctgtatcca ggggagtcat 1021 ttgcacacag tataaaaaca tggtttgaga gtgttcctgg aaaacaatgg aaaggacaat 1081 tcaccacagt ccgaaaaagt ggccagtgtt cgggctgtgg aaaaaccata gagtctattc 1141 agctgagtcc agaagaatat gaatgtctta agggaaaaat catgagggat gtgatagatg 1201 gaggtgacca gtacagaaag acaacacctc aggaacttaa gagatttgag aacttcataa 1261 aatctcgtcc tcCTTTTGAT GTTGTCATTG ATGGTCTCAA TGTTGCCAAA ATGTTTCCTA 1321 AAGTTCGTGA ATCTCAACTT CTCTTGAATG TCGTCTCTCA ACTAGCCAAA CGGAATCTGC 1381 GACTGCTGGT CCTAGGCCGG AAGCACATGC TAAGACGGAG TTCCCAGTGG AGTCGGGATG 1441 AGATGGAAGA GGTGCAAAAG CAAGCCAGCT GTTTTTTTGC TGATGACATC TCGGAGGATG 1501 ATCCATTCCT TCTGTATGCC ACACTGCACT CCGGGAATCA CTGCAGGTTT ATCACAAGAG 1561 ACCTGATGCG GGACCACAAG GCCTGTCTGC CTGATGCCAA GACCCAACGC CTGTTTTTTA 1621 AGTGGCAGCA GGGACATCAG CTGGCAATTG TAAATAGGTT TCCAGGATCA AAACTAACCT 1681 TTCAGCGTAT TCTCAGCTAT GACACAGTGG TGCAAACAAC TGGAGACTCG TGGCACATAC 1741 CATATGATGA AGACTTGGTA GAAAGATGTT CCTGTGAAGT ACCAACCAAA TGGCTTTGCC 1801 TCCACCAAAA GACATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1861 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1921 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGCGTTCCGC AAATACCAGA 1981 CGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt