Construct: ORF TRCN0000474471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003631.1_s317c1
- Derived from:
- ccsbBroadEn_01586
- DNA Barcode:
- CGGCTATATCGGAATACAATCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPINK1 (6690)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6690 | SPINK1 | serine peptidase inhibitor ... | NM_001354966.1 | 100% | 100% | |
| 2 | human | 6690 | SPINK1 | serine peptidase inhibitor ... | NM_003122.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 226
- ORF end:
- 463
- ORF length:
- 237
- Sequence:
-
1 GGGGGGAGGG GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA 61 GGCCAGCTTG GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT 121 GGTTCATTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT 181 GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG TTGGCATGAA GGTAACAGGC 241 ATCTTTCTTC TCAGTGCCTT GGCCCTGTTG AGTCTATCTG GTAACACTGG AGCTGACTCC 301 CTGGGAAGAG AGGCCAAATG TTACAATGAA CTTAATGGAT GCACCAAGAT ATATGACCCT 361 GTCTGTGGGA CTGATGGAAA TACTTATCCC AATGAATGCG TGTTATGTTT TGAAAATCGG 421 AAACGCCAGA CTTCTATCCT CATTCAAAAA TCTGGGCCTT GCTGCCCAAC TTTCTTGTAC 481 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 541 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 601 AAGGACGACG GCTATATCGG AATACAATCA AAACGCGTTA AGTCgacaat caacctctgg 661 attacaaaat ttgtgaaaga tt