Construct: ORF TRCN0000474491
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002682.1_s317c1
- Derived from:
- ccsbBroadEn_03338
- DNA Barcode:
- ATCATTTAGAACTAGGCTTATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AZIN1 (51582)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474491
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_001363024.1 | 100% | 100% | |
| 2 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_001363083.1 | 100% | 100% | |
| 3 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_015878.5 | 100% | 100% | |
| 4 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_148174.4 | 100% | 100% | |
| 5 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_001363010.1 | 99.7% | 99.7% | 902_903insAGT |
| 6 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_001363011.1 | 99.7% | 99.7% | 902_903insAGT |
| 7 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_001363012.1 | 99.7% | 99.7% | 902_903insAGT |
| 8 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_001301668.2 | 81% | 80.5% | 1083_1084ins25;1087_1088insTGA;1089_1090ins227 |
| 9 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_001363013.1 | 62.7% | 62.7% | 0_1ins501 |
| 10 | human | 51582 | AZIN1 | antizyme inhibitor 1 | NM_001363014.1 | 62.7% | 62.7% | 0_1ins501 |
| 11 | human | 51582 | AZIN1 | antizyme inhibitor 1 | XM_017013566.2 | 62.5% | 62.5% | 0_1ins501;401_402insAGT |
| 12 | mouse | 54375 | Azin1 | antizyme inhibitor 1 | NM_001102458.1 | 93.5% | 95.7% | (many diffs) |
| 13 | mouse | 54375 | Azin1 | antizyme inhibitor 1 | NM_018745.5 | 93.5% | 95.7% | (many diffs) |
| 14 | mouse | 54375 | Azin1 | antizyme inhibitor 1 | XM_006520114.3 | 93.3% | 95.5% | (many diffs) |
| 15 | mouse | 54375 | Azin1 | antizyme inhibitor 1 | NM_001301688.1 | 75% | 77.2% | (many diffs) |
| 16 | mouse | 54375 | Azin1 | antizyme inhibitor 1 | XM_006520117.1 | 58.9% | 60% | (many diffs) |
| 17 | mouse | 54375 | Azin1 | antizyme inhibitor 1 | NR_125913.1 | 24.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1410
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aggatttatt gatgatgcaa actactccgt tggcctgttg gatgaaggaa 121 caaaccttgg aaatgttatt gataactatg tttatgaaca taccctgaca gggaaaaatg 181 cattttttgt gggagatctt ggaaagattg tgaagaaaca cagtcaatgg cagaatgtag 241 tggctcagat aaagccattc tacacagtga agtgcaactc tgctccagct gtacttgaga 301 ttttggcagc tcttggaacc ggatttgctt gttccagtaa aaatgaaatg gctttagtgc 361 aagagttggg tgtacctcca gaaaacatta tttacataag tccttgcaag caagtgtctc 421 agataaagta tgcagcaaaa gttggagtga atatcctgac atgtgacaat gaaattgaat 481 tgaagaaaat tgcacgtaat cacccaaatg ccaaggtctt actacatatt gcaacagaag 541 ataatattgg aggtgaagag ggtaacatga agtttggcac taccctgaag aactgtaggc 601 atctcttgga atgtgctaag gaacttgatg tccaaataat tggggttaaa tttcatgttt 661 cgagtgcttg caaagaatct caagtatatg tacatgctct atctgatgct cgatgtgtgt 721 ttgacatggc tggagaaatt ggctttacga tgaacatgtt agacattggt ggaggattca 781 cgggaactga atttcaattg gaagaggtta atcatgttat cagccctctg ttggatatct 841 actttcctga aggatctggt gttaagataa tttcagaacc cggaagctac tatgtgtctt 901 ctgcatttac actcgcagtt aatatcatag caaagaaagt tgttgaaaat gataaatttc 961 cctctggagt agaaaaaacc ggaagtgatg aaccagcctt catgtattat atgaatgatg 1021 gtgtttatgg TTCTTTTGCA AGTAAACTGT CTGAGGACTT AAATACCATT CCAGAGGTTC 1081 ACAAGAAATA CAAGGAAGAT GAGCCTCTGT TTACAAGCAG CCTTTGGGGT CCATCCTGTG 1141 ATGAGCTTGA TCAAATTGTG GAAAGCTGTC TTCTTCCTGA GCTGAATGTG GGAGATTGGC 1201 TTATCTTTGA TAACATGGGA GCAGATTCTT TCCATGAACC ATCTGCTTTT AATGATTTTC 1261 AGAGGCCAGC CATTTATTAC ATGATGTCAT TCAGTGATTG GTATGAGATG CAAGATGCTG 1321 GAATTACTTC AGACTCAATG ATGAAGAACT TCTTCTTTGT GCCTTCTTGC ATTCAGCTGA 1381 GCCAAGAAGA CAGCTTTTCC GCTGAAGCTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1441 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1501 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCAT 1561 TTAGAACTAG GCTTATAATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1621 tgaaagatt