Construct: ORF TRCN0000474532

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018485.1_s317c1
Derived from:
ccsbBroadEn_02685
DNA Barcode:
ACTCTCGGCGATATCCCCTGCCTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
IKZF2 (22807)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474532
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22807 IKZF2 IKAROS family zinc finger 2 NM_001079526.2 99.9% 72.2% 1067_1068insC
2 human 22807 IKZF2 IKAROS family zinc finger 2 NM_001371275.1 99.9% 72.2% 1067_1068insC
3 human 22807 IKZF2 IKAROS family zinc finger 2 NM_001371276.1 99.9% 72.2% 1067_1068insC
4 human 22807 IKZF2 IKAROS family zinc finger 2 XM_005246386.5 98.7% 71.4% 1_18del;1085_1086insC
5 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510815.3 98.5% 71% 1_18del;53_55delCAG;1088_1089insC
6 human 22807 IKZF2 IKAROS family zinc finger 2 NM_001371274.1 94.9% 68.6% 327_404del;1145_1146insC
7 human 22807 IKZF2 IKAROS family zinc finger 2 NM_016260.3 94.9% 68.6% 327_404del;1145_1146insC
8 human 22807 IKZF2 IKAROS family zinc finger 2 XM_005246384.5 94.9% 68.6% 327_404del;1145_1146insC
9 human 22807 IKZF2 IKAROS family zinc finger 2 XM_005246385.4 94.9% 68.6% 327_404del;1145_1146insC
10 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510807.3 94.8% 68.3% 35_37delCAG;330_407del;1148_1149insC
11 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510808.3 94.8% 68.3% 35_37delCAG;330_407del;1148_1149insC
12 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510809.3 94.8% 68.3% 35_37delCAG;330_407del;1148_1149insC
13 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510810.3 94.8% 68.3% 35_37delCAG;330_407del;1148_1149insC
14 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510811.3 94.8% 68.3% 35_37delCAG;330_407del;1148_1149insC
15 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510812.3 94.8% 68.3% 35_37delCAG;330_407del;1148_1149insC
16 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510802.3 93.7% 67.6% (many diffs)
17 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510803.3 93.7% 67.6% (many diffs)
18 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510804.3 93.7% 67.6% (many diffs)
19 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510805.3 93.7% 67.6% (many diffs)
20 human 22807 IKZF2 IKAROS family zinc finger 2 NM_001371277.1 90.1% 62.4% 135_136insAGC;630_631ins144;920_921insC
21 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510819.3 89.2% 61.9% 1_18del;651_652ins144;941_942insC
22 human 22807 IKZF2 IKAROS family zinc finger 2 XM_017003592.2 89% 61.6% (many diffs)
23 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510816.3 88.4% 60.5% (many diffs)
24 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510818.3 84.9% 58.6% 0_1ins159;168_245del;986_987insC
25 human 22807 IKZF2 IKAROS family zinc finger 2 XM_017003591.2 84.9% 58.6% 0_1ins159;168_245del;986_987insC
26 human 22807 IKZF2 IKAROS family zinc finger 2 XM_011510817.3 84.7% 58.6% (many diffs)
27 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495968.2 91.2% 67.5% (many diffs)
28 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_011238509.2 91% 67.2% (many diffs)
29 mouse 22779 Ikzf2 IKAROS family zinc finger 2 NM_011770.4 90.7% 67.1% (many diffs)
30 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495966.3 90.7% 67.1% (many diffs)
31 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495967.3 90.7% 67.1% (many diffs)
32 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_017320399.1 90.7% 67.1% (many diffs)
33 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495962.3 90.5% 66.8% (many diffs)
34 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495963.3 90.5% 66.8% (many diffs)
35 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495964.2 90.5% 66.8% (many diffs)
36 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495965.3 90.5% 66.8% (many diffs)
37 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_011238508.2 90.5% 66.8% (many diffs)
38 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495961.2 89.7% 66.4% (many diffs)
39 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_011238505.2 89.5% 66.1% (many diffs)
40 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_011238506.2 89.5% 66.1% (many diffs)
41 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_006495959.2 86.9% 64.3% (many diffs)
42 mouse 22779 Ikzf2 IKAROS family zinc finger 2 XM_011238504.2 86.7% 64% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1194
ORF length:
1128
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga aacagaggct attgatggct atataacgtg tgacaatgag ctttcacccg
121aaagggagca ctccaatatg gcaattgacc tcacctcaag cacacccaat ggacagcatg
181cctcaccaag tcacatgaca agcacaaatt cagtaaagct agaaatgcag agtgatgaag
241agtgtgacag gaaacccctg agccgtgaag atgagatcag gggccatgat gagggtagca
301gcctagaaga acccctaatt gagagcagcg aggtggctga caacaggaaa gtccaggagc
361ttcaaggcga gggaggaatc cggcttccga atggtgaacg ccccttccac tgtaaccagt
421gtggagcttc ttttactcag aagggcaacc ttctgagaca cataaagtta cactctggag
481agaagccgtt caaatgtcct ttctgtagct acgcctgtag aagaagggac gccctcacag
541gacacctcag gacccattct gtgggtaaac ctcacaagtg caactactgt ggacgaagct
601acaagcagcg cagttcactg gaggagcaca aggaacgctg ccacaactat ctccagaatg
661tcagcatgga ggctgctggg caggtcatga gtcaccatgt acctcctatg gaagattgta
721aggaacaaga gcctattatg gacaacaata tttctctggt gccttttgag agacctgctg
781tcatagagaa gctcacgggg aatatgggaa aacgtaaaag ctccactcca caaaagtttg
841tgggggaaaa gctcatgcga ttcagctacc cagatattca ctttgatatg aacttaacat
901atgagaagga ggctgagctg atgcagtctc atatgatgga ccaagccatc aacaatgcaa
961tcacctacct tggagctgag gcccttcacc ctctgatgca gcacccgcca agcacaatcg
1021ctgaagtgGC CCCAGTTATA AGCTCAGCTT ATTCTCAGGT CTATCATCCA AATAGGATAG
1081AAAGACCCAT TAGCAGGGAA ACTGCTGATA GTCATGAAAA CAACATGGAT GGCCCCCATC
1141TCTCTCATCA GACCAAAGAG TCGACCCCAG GAAAGAGAGG CCTCTCCCAG CAATAGCTGC
1201CTGGATTCCA CTGACTCAGA AAGCAGCCAT GATGACCACC AGTCCTACCA AGGACACCCT
1261GCCTTAAATC CCAAGAGGAA ACAAAGCCCA GCTTACATGA AGGAGGATGT CAAAGCTTTG
1321GATACTACCA AGGCTCCTAA GGGCTCTCTG AAGGACATCT ACAAGGTCTT CAATGGAGAA
1381GGAGAACAGA TTAGGGCCTT CAAGTGTGAG CACTGCCGAG TCCTTTTCCT AGACCATGTC
1441ATGTACACCA TTCACATGGG TTGCCATGGC TACCGGGACC CACTGGAATG CAACATCTGT
1501GGCTACAGAA GCCAGGACCG TTATGAGTTT TCATCACACA TTGTTCGAGG GGAGCACACA
1561TTCCACAACC TAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT
1621CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT
1681TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACTCTCGG CGATATCCCC TGCCTCACGC
1741GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)