Construct: ORF TRCN0000474532
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018485.1_s317c1
- Derived from:
- ccsbBroadEn_02685
- DNA Barcode:
- ACTCTCGGCGATATCCCCTGCCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- IKZF2 (22807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474532
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | NM_001079526.2 | 99.9% | 72.2% | 1067_1068insC |
2 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | NM_001371275.1 | 99.9% | 72.2% | 1067_1068insC |
3 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | NM_001371276.1 | 99.9% | 72.2% | 1067_1068insC |
4 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_005246386.5 | 98.7% | 71.4% | 1_18del;1085_1086insC |
5 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510815.3 | 98.5% | 71% | 1_18del;53_55delCAG;1088_1089insC |
6 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | NM_001371274.1 | 94.9% | 68.6% | 327_404del;1145_1146insC |
7 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | NM_016260.3 | 94.9% | 68.6% | 327_404del;1145_1146insC |
8 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_005246384.5 | 94.9% | 68.6% | 327_404del;1145_1146insC |
9 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_005246385.4 | 94.9% | 68.6% | 327_404del;1145_1146insC |
10 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510807.3 | 94.8% | 68.3% | 35_37delCAG;330_407del;1148_1149insC |
11 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510808.3 | 94.8% | 68.3% | 35_37delCAG;330_407del;1148_1149insC |
12 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510809.3 | 94.8% | 68.3% | 35_37delCAG;330_407del;1148_1149insC |
13 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510810.3 | 94.8% | 68.3% | 35_37delCAG;330_407del;1148_1149insC |
14 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510811.3 | 94.8% | 68.3% | 35_37delCAG;330_407del;1148_1149insC |
15 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510812.3 | 94.8% | 68.3% | 35_37delCAG;330_407del;1148_1149insC |
16 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510802.3 | 93.7% | 67.6% | (many diffs) |
17 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510803.3 | 93.7% | 67.6% | (many diffs) |
18 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510804.3 | 93.7% | 67.6% | (many diffs) |
19 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510805.3 | 93.7% | 67.6% | (many diffs) |
20 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | NM_001371277.1 | 90.1% | 62.4% | 135_136insAGC;630_631ins144;920_921insC |
21 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510819.3 | 89.2% | 61.9% | 1_18del;651_652ins144;941_942insC |
22 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_017003592.2 | 89% | 61.6% | (many diffs) |
23 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510816.3 | 88.4% | 60.5% | (many diffs) |
24 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510818.3 | 84.9% | 58.6% | 0_1ins159;168_245del;986_987insC |
25 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_017003591.2 | 84.9% | 58.6% | 0_1ins159;168_245del;986_987insC |
26 | human | 22807 | IKZF2 | IKAROS family zinc finger 2 | XM_011510817.3 | 84.7% | 58.6% | (many diffs) |
27 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495968.2 | 91.2% | 67.5% | (many diffs) |
28 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_011238509.2 | 91% | 67.2% | (many diffs) |
29 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | NM_011770.4 | 90.7% | 67.1% | (many diffs) |
30 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495966.3 | 90.7% | 67.1% | (many diffs) |
31 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495967.3 | 90.7% | 67.1% | (many diffs) |
32 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_017320399.1 | 90.7% | 67.1% | (many diffs) |
33 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495962.3 | 90.5% | 66.8% | (many diffs) |
34 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495963.3 | 90.5% | 66.8% | (many diffs) |
35 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495964.2 | 90.5% | 66.8% | (many diffs) |
36 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495965.3 | 90.5% | 66.8% | (many diffs) |
37 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_011238508.2 | 90.5% | 66.8% | (many diffs) |
38 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495961.2 | 89.7% | 66.4% | (many diffs) |
39 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_011238505.2 | 89.5% | 66.1% | (many diffs) |
40 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_011238506.2 | 89.5% | 66.1% | (many diffs) |
41 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_006495959.2 | 86.9% | 64.3% | (many diffs) |
42 | mouse | 22779 | Ikzf2 | IKAROS family zinc finger 2 | XM_011238504.2 | 86.7% | 64% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aacagaggct attgatggct atataacgtg tgacaatgag ctttcacccg 121 aaagggagca ctccaatatg gcaattgacc tcacctcaag cacacccaat ggacagcatg 181 cctcaccaag tcacatgaca agcacaaatt cagtaaagct agaaatgcag agtgatgaag 241 agtgtgacag gaaacccctg agccgtgaag atgagatcag gggccatgat gagggtagca 301 gcctagaaga acccctaatt gagagcagcg aggtggctga caacaggaaa gtccaggagc 361 ttcaaggcga gggaggaatc cggcttccga atggtgaacg ccccttccac tgtaaccagt 421 gtggagcttc ttttactcag aagggcaacc ttctgagaca cataaagtta cactctggag 481 agaagccgtt caaatgtcct ttctgtagct acgcctgtag aagaagggac gccctcacag 541 gacacctcag gacccattct gtgggtaaac ctcacaagtg caactactgt ggacgaagct 601 acaagcagcg cagttcactg gaggagcaca aggaacgctg ccacaactat ctccagaatg 661 tcagcatgga ggctgctggg caggtcatga gtcaccatgt acctcctatg gaagattgta 721 aggaacaaga gcctattatg gacaacaata tttctctggt gccttttgag agacctgctg 781 tcatagagaa gctcacgggg aatatgggaa aacgtaaaag ctccactcca caaaagtttg 841 tgggggaaaa gctcatgcga ttcagctacc cagatattca ctttgatatg aacttaacat 901 atgagaagga ggctgagctg atgcagtctc atatgatgga ccaagccatc aacaatgcaa 961 tcacctacct tggagctgag gcccttcacc ctctgatgca gcacccgcca agcacaatcg 1021 ctgaagtgGC CCCAGTTATA AGCTCAGCTT ATTCTCAGGT CTATCATCCA AATAGGATAG 1081 AAAGACCCAT TAGCAGGGAA ACTGCTGATA GTCATGAAAA CAACATGGAT GGCCCCCATC 1141 TCTCTCATCA GACCAAAGAG TCGACCCCAG GAAAGAGAGG CCTCTCCCAG CAATAGCTGC 1201 CTGGATTCCA CTGACTCAGA AAGCAGCCAT GATGACCACC AGTCCTACCA AGGACACCCT 1261 GCCTTAAATC CCAAGAGGAA ACAAAGCCCA GCTTACATGA AGGAGGATGT CAAAGCTTTG 1321 GATACTACCA AGGCTCCTAA GGGCTCTCTG AAGGACATCT ACAAGGTCTT CAATGGAGAA 1381 GGAGAACAGA TTAGGGCCTT CAAGTGTGAG CACTGCCGAG TCCTTTTCCT AGACCATGTC 1441 ATGTACACCA TTCACATGGG TTGCCATGGC TACCGGGACC CACTGGAATG CAACATCTGT 1501 GGCTACAGAA GCCAGGACCG TTATGAGTTT TCATCACACA TTGTTCGAGG GGAGCACACA 1561 TTCCACAACC TAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT 1621 CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT 1681 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACTCTCGG CGATATCCCC TGCCTCACGC 1741 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt