Construct: ORF TRCN0000474539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014424.1_s317c1
- Derived from:
- ccsbBroadEn_06110
- DNA Barcode:
- GTATGAACGGGGAGATCAGTGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC1I1 (1780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474539
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001135556.1 | 99.8% | 99.8% | 675A>G;997C>G;1440C>A |
| 2 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_004411.4 | 97.2% | 97.2% | (many diffs) |
| 3 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001135557.1 | 96.6% | 96.6% | (many diffs) |
| 4 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001278421.1 | 94.1% | 94.1% | (many diffs) |
| 5 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001278422.1 | 91.8% | 89.7% | (many diffs) |
| 6 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_010063.4 | 91.2% | 97.9% | (many diffs) |
| 7 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191026.1 | 89.5% | 96.1% | (many diffs) |
| 8 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | XM_017321380.1 | 89.5% | 96.1% | (many diffs) |
| 9 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | XM_006504982.3 | 88.8% | 95.3% | (many diffs) |
| 10 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191025.1 | 88.1% | 94.7% | (many diffs) |
| 11 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191027.1 | 86.4% | 92.9% | (many diffs) |
| 12 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191023.1 | 85.7% | 92.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1950
- ORF length:
- 1884
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgacaaaagt gacttaaaag ctgagctaga gcgcaaaaag cagcgcttag 121 cacagataag agaagagaag aaacggaagg aagaggagag gaaaaagaaa gaggctgata 181 tgcagcagaa gaaagaaccc gttcaggacg actctgatct ggatcgcaaa cgacgagaga 241 cagaggcttt gctgcaaagc attggtatct caccggagcc gcctctagtc ccaaccccta 301 tgtctccctc ctcgaaatca gtgagcactc ccagtgaagc tggaagccaa gactcaggcg 361 atctggggcc attaacaagg accctgcagt gggacacaga cccctcagtg ctccagctgc 421 agtcagactc agaacttgga agaagactgc ataaactggg cgtgtcaaag gtcacccaag 481 tggatttcct gccaagggaa gtagtgtcct actcaaagga gacccagact cctcttgcca 541 cgcatcagtc tgaagaggat gaggaagatg aggaaatggt ggaatctaaa gttggccagg 601 actcagaact ggaaaatcag gacaaaaaac aggaagtgaa ggaagcccct ccaagagagt 661 tgacagagga agaaaaacag cagatccttc attcagagga atttctcatc ttttttgacc 721 ggacaatacg ggtaattgag agagccctgg ctgaagattc cgacatcttt tttgactaca 781 gcggccgaga gttagaggaa aaagatgggg atgttcaggc tggagccaat ctttctttca 841 atcgtcagtt ctatgatgaa cattggtcca agcatcgagt ggtcacttgt atggactggt 901 ccctccagta ccctgagctg atggtggctt cttacaacaa caatgaagat gctccccatg 961 aaccagatgg agtggccttg gtttggaaca tgaagtttaa gaaaaccaca ccagaatacg 1021 tcttccactg tcagtcctct gtgatgtcgg tctgcttcgc cggtttccat cctaacttgg 1081 tggttggtgg gacttactcg ggccagattg tcctctggga caatcgcagt catcgaagga 1141 ctccagtgca gcggacaccc ttatcagctg ctgcacacac gcatcccgtg tactgtgtaa 1201 atgttgttgg gacccagaat gctcataacc tcatcactgt ctccactgat ggcaaaatgt 1261 gttcctggag cctggacatg ctctcaactc cacaggagag catggagctg gtgtacaata 1321 agtccaagcc tgtcgctgtt accggaatgg ctttcccaac gggagacgtc aataacttcg 1381 tggttggcag tgaggaaggt acagtctaca cggcttgtcg tcatggaagc aaagcaggta 1441 ttggtgaggt ctttgaaggT CACCAAGGGC CAGTGACAGG AATTAACTGC CACATGGCAG 1501 TGGGACCAAT CGACTTTTCT CACCTGTTTG TCACATCATC ATTTGACTGG ACTGTCAAAC 1561 TGTGGACCAC CAAGCACAAC AAGCCGCTCT ACTCCTTTGA AGACAATGCA GACTATGTGT 1621 ACGATGTCAT GTGGTCCCCC GTGCATCCTG CGCTTTTTGC CTGCGTGGAC GGGATGGGGC 1681 GCTTGGACCT CTGGAACCTC AACAATGACA CCGAGGTTCC AACAGCAAGT GTGGCCATTG 1741 AGGGGGCATC CGCCCTAAAC CGTGTTCGTT GGGCCCAAGC TGGCAAAGAA GTTGCTGTTG 1801 GGGACTCGGA AGGCCGTATT TGGGTCTATG ACGTTGGAGA GCTTGCAGTT CCCCACAATG 1861 ATGAATGGAC CCGATTTGCC AGGACCCTTG TGGAAATTCG TGCTAACAGA GCTGATAGCG 1921 AGGAGGAAGG CACTGTTGAG TTATCTGCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1981 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2041 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGTATG 2101 AACGGGGAGA TCAGTGTACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2161 tgaaagatt