Construct: ORF TRCN0000474550
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012289.1_s317c1
- Derived from:
- ccsbBroadEn_00165
- DNA Barcode:
- GTACCTAGACTTTCTACTCCCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMI1 (648)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474550
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 648 | BMI1 | BMI1 proto-oncogene, polyco... | NM_005180.9 | 100% | 100% | |
| 2 | human | 100532731 | COMMD3-BMI1 | COMMD3-BMI1 readthrough | NM_001204062.1 | 69.5% | 69.5% | 1_429del |
| 3 | mouse | 12151 | Bmi1 | Bmi1 polycomb ring finger o... | NM_007552.4 | 92% | 96.9% | (many diffs) |
| 4 | mouse | 12151 | Bmi1 | Bmi1 polycomb ring finger o... | XM_006497311.2 | 92% | 96.9% | (many diffs) |
| 5 | mouse | 12151 | Bmi1 | Bmi1 polycomb ring finger o... | XM_006497312.3 | 92% | 96.9% | (many diffs) |
| 6 | mouse | 546387 | Gm5946 | predicted gene 5946 | XR_387091.3 | 36% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tcgaacaacg agaatcaaga tcactgagct aaatccccac ctgatgtgtg 121 tgctttgtgg agggtacttc attgatgcca caaccataat agaatgtcta cattccttct 181 gtaaaacgtg tattgttcgt tacctggaga ccagcaagta ttgtcctatt tgtgatgtcc 241 aagttcacaa gaccagacca ctactgaata taaggtcaga taaaactctc caagatattg 301 tatacaaatt agttccaggg cttttcaaaa atgaaatgaa gagaagaagg gatttttatg 361 cagctcatcc ttctgctgat gctgccaatg gctctaatga agatagagga gaggttgcag 421 atgaagataa gagaattata actgatgatg agataataag cttatccatt gaattctttg 481 accagaacag attggatcGG AAAGTAAACA AAGACAAAGA GAAATCTAAG GAGGAGGTGA 541 ATGATAAAAG ATACTTACGA TGCCCAGCAG CAATGACTGT GATGCACTTA AGAAAGTTTC 601 TCAGAAGTAA AATGGACATA CCTAATACTT TCCAGATTGA TGTCATGTAT GAGGAGGAAC 661 CTTTAAAGGA TTATTATACA CTAATGGATA TTGCCTACAT TTATACCTGG AGAAGGAATG 721 GTCCACTTCC ATTGAAATAC AGAGTTCGAC CTACTTGTAA AAGAATGAAG ATCAGTCACC 781 AGAGAGATGG ACTGACAAAT GCTGGAGAAC TGGAAAGTGA CTCTGGGAGT GACAAGGCCA 841 ACAGCCCAGC AGGAGGTATT CCCTCCACCT CTTCTTGTTT GCCTAGCCCC AGTACTCCAG 901 TGCAGTCTCC TCATCCACAG TTTCCTCACA TTTCCAGTAC TATGAATGGA ACCAGCAACA 961 GCCCCAGCGG TAACCACCAA TCTTCTTTTG CCAATAGACC TCGAAAATCA TCAGTAAATG 1021 GGTCATCAGC AACTTCTTCT GGTTGCCTAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TACCTAGACT 1201 TTCTACTCCC TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att