Construct: ORF TRCN0000474566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013340.1_s317c1
- Derived from:
- ccsbBroadEn_11970
- DNA Barcode:
- CGAAATGTGACATTCACGCAGCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ERGIC2 (51290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51290 | ERGIC2 | ERGIC and golgi 2 | NM_016570.3 | 56.8% | 19.3% | (many diffs) |
| 2 | human | 51290 | ERGIC2 | ERGIC and golgi 2 | XM_024449009.1 | 56.8% | 19.3% | (many diffs) |
| 3 | human | 51290 | ERGIC2 | ERGIC and golgi 2 | XR_001748740.2 | 13.9% | (many diffs) | |
| 4 | human | 51290 | ERGIC2 | ERGIC and golgi 2 | XR_001748741.2 | 12.8% | (many diffs) | |
| 5 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XM_017321718.1 | 87.5% | 32.5% | (many diffs) |
| 6 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XM_011241623.2 | 66.2% | 24.3% | (many diffs) |
| 7 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XM_017321717.1 | 64% | 23.5% | (many diffs) |
| 8 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XM_006507089.3 | 52.7% | 19.3% | (many diffs) |
| 9 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | NM_026168.4 | 51.3% | 18.8% | (many diffs) |
| 10 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XM_006507087.2 | 51.3% | 18.8% | (many diffs) |
| 11 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XM_017321716.1 | 51.3% | 18.8% | (many diffs) |
| 12 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XR_001785162.1 | 37.8% | (many diffs) | |
| 13 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | NM_001286560.1 | 31.3% | 4% | (many diffs) |
| 14 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XR_378169.3 | 18.1% | (many diffs) | |
| 15 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XR_001785161.1 | 17.4% | (many diffs) | |
| 16 | mouse | 67456 | Ergic2 | ERGIC and golgi 2 | XR_378170.3 | 13.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 294
- ORF length:
- 228
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcgactgaat cggaaaaaaa ctttaagttt ggtaaaagag ttggatgcct 121 ttccgaaggt tcctgagagc tatgtagaga cttcagccag tggaggtaca gtttctctaa 181 tagcatttac aactatggct ttattaacca taatggaatt ctcagtatat caAGATACAT 241 GGATGAAGTA TGAATACGAA GTAGACAAGG ATTTTTCTAG CAAAATTAAG AATTAATATA 301 GATATTACCG TTGCCATGAA GTGTCAATAT GTTGGAGCGG ATGTATTGGA TTTAGCAGAA 361 ACAATGTTTG CATCTGCAGA TGGTTTAGTT TATGAACCAA CAGTATTTGA TCTTTCACCA 421 CAGCAGAAAG AGTGGCAGAG GATGCTGCAG CTGATTCAGA GTAGGCTACA AGAAGAGCAT 481 TCACTTCAAG ATGTGATATT TAAAAGTGCT TTTAAAAGTA CATCAACAGC TCTTCCACCA 541 AGAGAAGATG ATTCATCACA GTCTCCAAAT GCATGCAGAA TTCATGGCCA TCTATATGTC 601 AATAAAGTAG CAGGGAATTT TCACATAACA GTGGGGCAAT TCCACATCCT CGTGGTCATG 661 CACATTTGGC AGCACTTGTC AACCATGAAT CTTACAATTT TTCTCATAGA ATACCCAACT 721 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 781 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 841 TCTTGTGGAA AGGACGACGA AATGTGACAT TCACGCAGCC GACGCGTTAA GTCgacaatc 901 aacctctgga ttacaaaatt tgtgaaagat t