Construct: ORF TRCN0000474570
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011141.1_s317c1
- Derived from:
- ccsbBroadEn_11822
- DNA Barcode:
- CGTTCATGGGTCCAAATAAACAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL3 (26249)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474570
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26249 | KLHL3 | kelch like family member 3 | NM_017415.3 | 51.2% | 51.2% | 471A>G;904_1761del |
| 2 | human | 26249 | KLHL3 | kelch like family member 3 | NM_001257194.1 | 45.7% | 45.8% | 0_1ins96;375A>G;808_1665del |
| 3 | human | 26249 | KLHL3 | kelch like family member 3 | NM_001257195.1 | 37.2% | 37.3% | 0_1ins246;225A>G;658_1515del |
| 4 | mouse | 100503085 | Klhl3 | kelch-like 3 | XM_006517009.2 | 46.1% | 50.7% | (many diffs) |
| 5 | mouse | 100503085 | Klhl3 | kelch-like 3 | XM_006517008.2 | 43.5% | 47.9% | (many diffs) |
| 6 | mouse | 100503085 | Klhl3 | kelch-like 3 | NM_001195075.1 | 40.7% | 44.5% | (many diffs) |
| 7 | mouse | 100503085 | Klhl3 | kelch-like 3 | XM_006517010.3 | 40.2% | 45.3% | (many diffs) |
| 8 | mouse | 100503085 | Klhl3 | kelch-like 3 | XM_006517011.3 | 40.2% | 45.3% | (many diffs) |
| 9 | mouse | 100503085 | Klhl3 | kelch-like 3 | XM_006517013.3 | 40.2% | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 969
- ORF length:
- 903
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggtgaaagt gtcaagctga gctcccagac tctgatacag gctggggatg 121 atgagaagaa ccagaggacg atcactgtca accctgccca catggggaaa gcattcaagg 181 ttatgaatga actgcggagt aaacagctgt tgtgtgacgt gatgattgtg gcagaagatg 241 tcgagataga agcccaccgt gtggtcctgg cagcctgcag cccctacttc tgtgcgatgt 301 tcacaggtga catgtctgag agtaaagcca aaaagataga aatcaaggac gtggatgggc 361 agacgctgag taagctgatt gactacatct atactgctga aatcgaggtg actgaagaga 421 atgtccaggt gctgctcccg gcagccagct tgctgcagct catggatgtt cggcagaact 481 gctgtgactt cctgcagtct cagttgcatc ccaccaattg cctgggcatc cgtgcgtttg 541 cagatgtaca cacctgcact gaccttctgc agcaggccaa tgccTACGCA GAGCAGCACT 601 TTCCAGAGGT GATGCTAGGA GAAGAATTTC TTAGCCTGAG TCTGGACCAG GTGTGCAGCT 661 TGATATCCAG CGACAAGCTG ACCGTTTCTT CAGAAGAGAA GGTGTTTGAA GCTGTGATCT 721 CATGGATCAA TTATGAGAAA GAAACCCGTT TAGAGCACAT GGCAAAGCTG ATGGAACATG 781 TCCGACTTCC TCTCTTACCT AGGGACTACC TAGTCCAAAC GGTTGAAGAA GAAGCTTTGA 841 TAAAGAATAA CAACACCTGT AAAGACTTCC TCATTGAGGC CATGAAATAC CATCTCCTCC 901 CTCTGGATCA GAGACTATTG ATTAAGAACC CAAGGACCAA GCCCAGGACT CCAGTCAGCC 961 TTCCCAAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1021 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1081 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGTTCA TGGGTCCAAA TAAACAGAAC 1141 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt