Transcript: Human NM_001257194.1

Homo sapiens kelch like family member 3 (KLHL3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-05
Taxon:
Homo sapiens (human)
Gene:
KLHL3 (26249)
Length:
6981
CDS:
716..2383

Additional Resources:

NCBI RefSeq record:
NM_001257194.1
NBCI Gene record:
KLHL3 (26249)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001257194.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274918 TATGCCGTGGGAGGGTTTAAT pLKO_005 1667 CDS 100% 15.000 21.000 N KLHL3 n/a
2 TRCN0000150648 GCTTTGCTAATTCACAGGTAA pLKO.1 3751 3UTR 100% 4.950 6.930 N KLHL3 n/a
3 TRCN0000285266 ATGCAGGTGTTGCCGTGATTC pLKO_005 2349 CDS 100% 10.800 8.640 N KLHL3 n/a
4 TRCN0000274864 ATGAATGAACTGCGGAGTAAA pLKO_005 737 CDS 100% 13.200 9.240 N KLHL3 n/a
5 TRCN0000155397 CAGCGACCAATGAATGGATAT pLKO.1 2013 CDS 100% 10.800 7.560 N KLHL3 n/a
6 TRCN0000158024 CTCAGTTGCATCCCACCAATT pLKO.1 1053 CDS 100% 10.800 7.560 N KLHL3 n/a
7 TRCN0000281958 GGGACCCTGAGACTACTAAAG pLKO_005 2841 3UTR 100% 10.800 7.560 N KLHL3 n/a
8 TRCN0000155462 GCAGAAGATGTCGAGATAGAA pLKO.1 785 CDS 100% 5.625 3.938 N KLHL3 n/a
9 TRCN0000157655 GCTTCGGTGGAGTACTACAAT pLKO.1 2273 CDS 100% 5.625 3.938 N KLHL3 n/a
10 TRCN0000158278 CCAGCGACCAATGAATGGATA pLKO.1 2012 CDS 100% 4.950 3.465 N KLHL3 n/a
11 TRCN0000154637 CCTGGAAGAAATGTCTCCAAA pLKO.1 4301 3UTR 100% 4.950 3.465 N KLHL3 n/a
12 TRCN0000157000 GCAGTAAATGGGCTCCTGTAT pLKO.1 2219 CDS 100% 4.950 3.465 N KLHL3 n/a
13 TRCN0000157154 GTGGCAGAAGATGTCGAGATA pLKO.1 782 CDS 100% 4.950 3.465 N KLHL3 n/a
14 TRCN0000274916 GTGGCAGAAGATGTCGAGATA pLKO_005 782 CDS 100% 4.950 3.465 N KLHL3 n/a
15 TRCN0000154581 GCTGTGATCTCATGGATCAAT pLKO.1 1265 CDS 100% 0.563 0.394 N KLHL3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001257194.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11822 pDONR223 100% 45.7% 45.8% None 0_1ins96;375A>G;808_1665del n/a
2 ccsbBroad304_11822 pLX_304 0% 45.7% 45.8% V5 0_1ins96;375A>G;808_1665del n/a
3 TRCN0000474570 CGTTCATGGGTCCAAATAAACAGA pLX_317 42.7% 45.7% 45.8% V5 0_1ins96;375A>G;808_1665del n/a
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