Construct: ORF TRCN0000474577
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012040.1_s317c1
- Derived from:
- ccsbBroadEn_14029
- DNA Barcode:
- CACCTTTATCTCTCCGTTTTCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RIMS3 (9783)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474577
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9783 | RIMS3 | regulating synaptic membran... | NM_014747.3 | 99.8% | 98% | 9_10insG |
| 2 | human | 9783 | RIMS3 | regulating synaptic membran... | XM_011542479.2 | 82.3% | 72.2% | (many diffs) |
| 3 | mouse | 242662 | Rims3 | regulating synaptic membran... | NM_182929.2 | 89.9% | 95.8% | (many diffs) |
| 4 | mouse | 242662 | Rims3 | regulating synaptic membran... | XM_006503101.1 | 89.9% | 95.8% | (many diffs) |
| 5 | mouse | 242662 | Rims3 | regulating synaptic membran... | XM_006503102.1 | 89.9% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 58
- ORF end:
- 991
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaatg 61 ttggcatgtt taacggggga gccaggtcct gcctcatctg gggcctccag gaatgtggtg 121 cggagctcca gcattagcgg tgaaatctgc ggatcccagc aagccggggg cggggctggg 181 accaccaccg ccaagaagcg gcggagcagc ctgggtgcca agatggtggc catcgtgggc 241 ctgactcagt ggagcaagag cacactccag cttccgcagc ctgaaggggc caccaagaag 301 ctgcgcagca acatccgccg gagcacggag acaggcatcg cggtggagat gcggagccgg 361 gtcacacgcc agggcagccg ggagtccacc gatgggagca ccaacagcaa cagctccgac 421 ggcacgttca tcttccccac tacccggcta ggggctgaaa gccagttcag cgatttcctg 481 gatgggctgg gaccagctca gattgtgggg cgacagacac tggcaacacc acccatggga 541 gatgtgcaca ttgccatcat ggaccggagt ggccagctgg aggtggaagt gattgaagct 601 cggggcctga cccccaaacc aggctccaaa tccctcccag ccacctatat caaggtttac 661 ctgctggaga atggggccTG CTTGGCCAAG AAGAAGACAA AGATGACCAA GAAGACCTGT 721 GATCCCCTGT ACCAGCAGGC TCTGCTCTTT GACGAGGGAC CCCAGGGCAA GGTGCTGCAG 781 GTGATCGTCT GGGGAGACTA TGGCCGCATG GACCACAAGT GCTTCATGGG CATGGCCCAG 841 ATCATGCTGG ACGAGCTGGA CCTCAGCGCC GCGGTCACCG GCTGGTACAA ACTCTTCCCC 901 ACCTCCTCAG TGGCAGACTC CACACTCGGA TCCCTCACCA GGCGCCTGTC CCAGTCTTCC 961 CTGGAGAGTG CCACCAGCCC CTCATGCTCT TACCCAACTT TCTTGTACAA AGTGGTTGAT 1021 ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT 1081 CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACACC 1141 TTTATCTCTC CGTTTTCGGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt 1201 gtgaaagatt