Construct: ORF TRCN0000474583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010479.1_s317c1
- Derived from:
- ccsbBroadEn_01458
- DNA Barcode:
- AACTTTCATTGAATTCTGGCGTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPS20 (6224)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474583
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6224 | RPS20 | ribosomal protein S20 | NM_001023.3 | 99.7% | 99.1% | 167T>A |
| 2 | human | 6224 | RPS20 | ribosomal protein S20 | NM_001146227.2 | 80.3% | 79.5% | (many diffs) |
| 3 | human | 54968 | TMEM70 | transmembrane protein 70 | NR_033334.1 | 14.4% | (many diffs) | |
| 4 | mouse | 67427 | Rps20 | ribosomal protein S20 | NM_026147.6 | 89.3% | 99.1% | (many diffs) |
| 5 | mouse | 625603 | Gm6607 | predicted gene 6607; 40S ri... | NR_033622.2 | 47.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 cagccatggc ttttaaggat accggaaaaa cacccgtgga gccggaggtg gcaattcacc 121 gaattcgaaT CACCCTAACA AGCCGCAACG TAAAATCCTT GGAAAAGGTG TGTGCTGACT 181 TGATAAGAGG CGCAAAAGAA AAGAATCTCA AAGTGAAAGG ACCAGTTCGA AAGCCTACCA 241 AGACTTTGAG AATCACTACA AGAAAAACTC CTTGTGGTGA AGGTTCTAAG ACGTGGGATC 301 GTTTCCAGAT GAGAATTCAC AAGCGACTCA TTGACTTGCA CAGTCCTTCT GAGATTGTTA 361 AGCAGATTAC TTCCATCAGT ATTGAGCCAG GAGTTGAGGT GGAAGTCACC ATTGCAGATG 421 CTTAAGTCAA CTATTTTAAT AAATTGATGA CCAGTTGTTA AAAAAAAAAA AAAAAAACTC 481 GAGGCATCTA TGTCGGGTGC GGAGAAAGAG GTAATGAAAT GGCACATGGT CATAGCTGTT 541 TCCTGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 601 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 661 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACTTTCATT GAATTCTGGC GTGCACGCGT 721 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt