Construct: ORF TRCN0000474604
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015880.1_s317c1
- Derived from:
- ccsbBroadEn_12199
- DNA Barcode:
- AGAAGAAAATGCAGTGCTGTTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM40 (55287)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474604
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55287 | TMEM40 | transmembrane protein 40 | NM_001284407.2 | 100% | 100% | |
| 2 | human | 55287 | TMEM40 | transmembrane protein 40 | NM_018306.4 | 87.1% | 87.1% | 211_300del |
| 3 | human | 55287 | TMEM40 | transmembrane protein 40 | XM_011533937.2 | 87.1% | 87.1% | 211_300del |
| 4 | human | 55287 | TMEM40 | transmembrane protein 40 | NM_001284406.1 | 81.5% | 81.5% | 1_48del;259_348del |
| 5 | human | 55287 | TMEM40 | transmembrane protein 40 | NM_001284408.2 | 77.3% | 76.8% | 73_74ins138 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 675
- ORF length:
- 609
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacttcagca tcctcctccc agcctcagga caacagtcaa gtccacagag 121 aaacagaaga tgtagactat ggagagacag atttccacaa gcaagatggg aaggctggac 181 tcttttccca agaacaatat gagagaaaca agtcttcttc ctcctcctcc tcttcctcct 241 catcctcctc atcttcttca tcctcctcct cctcaggtcc tgggcatggg gagcctgacg 301 ttttgaagga tgagcttcaa ctctatggag atgctcctgg agaggtggta ccctctgggG 361 AATCAGGACT CCGAAGGAGA GGCTCTGACC CAGCAAGTGG AGAAGTGGAG GCCTCTCAGT 421 TAAGAAGACT GAATATAAAG AAAGATGATG AGTTTTTCCA TTTCGTCCTC CTGTGCTTTG 481 CCATCGGGGC CTTGCTGGTG TGTTATCACT ATTACGCAGA CTGGTTCATG TCTCTTGGGG 541 TCGGCCTGCT CACCTTCGCC TCCCTGGAAA CCGTTGGCAT CTACTTCGGA CTAGTGTACC 601 GTATCCACAG CGTCCTCCAA GGCTTCATCC CCCTCTTCCA GAAGTTTAGG CTGACAGGGT 661 TCAGGAAGAC TGACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 721 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 781 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGAAGAAAAT GCAGTGCTGT 841 TGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt