Construct: ORF TRCN0000474610
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010789.1_s317c1
- Derived from:
- ccsbBroadEn_06710
- DNA Barcode:
- ATAAAACGCCTACGGAACGTTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPINF1 (5176)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474610
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5176 | SERPINF1 | serpin family F member 1 | NM_001329903.1 | 99.8% | 100% | 390T>C;963T>C |
| 2 | human | 5176 | SERPINF1 | serpin family F member 1 | NM_002615.7 | 99.8% | 100% | 390T>C;963T>C |
| 3 | human | 5176 | SERPINF1 | serpin family F member 1 | NM_001329904.1 | 55.1% | 55.2% | 0_1ins561;402T>C |
| 4 | human | 5176 | SERPINF1 | serpin family F member 1 | NM_001329905.1 | 55.1% | 55.2% | 0_1ins561;402T>C |
| 5 | mouse | 20317 | Serpinf1 | serine (or cysteine) peptid... | NM_011340.3 | 83.7% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggccctggtg ctactcctct gcattggagc cctcctcggg cacagcagct 121 gccagaaccc tgccagcccc ccggaggagg gctccccaga ccccgacagc acaggggcgc 181 tggtggagga ggaggatcct ttcttcaaag tccccgtgaa caagctggca gcggctgtct 241 ccaacttcgg ctatgacctg taccgggtgc gatccagcac gagccccacg accaacgtgc 301 tcctgtctcc tctcagtgtg gccacggccc tctcggccct ctcgctggga gcggagcagc 361 gaacagaatc catcattcac cgggctctct actatgactt gatcagcagc ccagacatcc 421 atggtaccta taaggagctc cttgacacgg tcaccgcccc ccagaagaac ctcaagagtg 481 cctcccggat cgtctttgag aagaagctgc gcataaaatc cagctttgtg gcacctctgg 541 aaaagtcata tgggaccagg cccagagtcc tgacgggcaa ccctcgcttg gacctgcaag 601 agatcaacaa ctgggtgcag gcgcagatga aagggaagct cgccaggtcc acaaaggaaa 661 ttcccgatga gatcagcatt ctccttctcg gtgtggcgca cttcaagggg cagtgggtaa 721 caaagtttga ctccagaaag acttccctcg aggatttcta cttggatgaa gagaggaccg 781 tgagggtccc catgatgtcg gaccctaagg ctgttttacg ctatggcttg gattcagatc 841 tcagctgcaa gattgcccag ctgcccttga ccggaagcat gagtatcatc ttcttcctgc 901 cccTGAAAGT GACCCAGAAT TTGACCTTGA TAGAGGAGAG CCTCACCTCC GAGTTCATTC 961 ATGACATAGA CCGAGAACTG AAGACCGTGC AGGCGGTCCT CACTGTCCCC AAGCTGAAGC 1021 TGAGTTACGA AGGCGAAGTC ACCAAGTCCC TGCAGGAGAT GAAGCTGCAA TCCTTGTTTG 1081 ATTCACCAGA CTTTAGCAAG ATCACAGGCA AACCCATCAA GCTGACTCAG GTGGAACACC 1141 GGGCTGGCTT TGAGTGGAAC GAGGATGGGG CGGGAACCAC CCCCAGCCCA GGGCTGCAGC 1201 CTGCCCACCT CACCTTCCCG CTGGACTATC ACCTTAACCA GCCTTTCATC TTCGTACTGA 1261 GGGACACAGA CACAGGGGCC CTTCTCTTCA TTGGCAAGAT TCTGGACCCC AGGGGCCCCT 1321 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGAATAAA ACGCCTACGG AACGTTAGCA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt