Construct: ORF TRCN0000474613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008976.1_s317c1
- Derived from:
- ccsbBroadEn_03752
- DNA Barcode:
- CGTCCGTAGTGTATGAGATGCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNO1 (56902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474613
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56902 | PNO1 | partner of NOB1 homolog | NM_020143.4 | 100% | 100% | |
| 2 | human | 56902 | PNO1 | partner of NOB1 homolog | NM_001329916.1 | 87.6% | 83.3% | 620_621ins71;663_664ins22 |
| 3 | human | 56902 | PNO1 | partner of NOB1 homolog | NM_001329917.2 | 83.9% | 83.7% | (many diffs) |
| 4 | human | 56902 | PNO1 | partner of NOB1 homolog | NR_138146.1 | 31.5% | 1_105del;606_607ins31;831_2269del | |
| 5 | mouse | 66249 | Pno1 | partner of NOB1 homolog | NM_025443.2 | 86.1% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 823
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgggcatgg aatccgaaat ggaaacgcag agcgccaggg cagaggaggg ctttacccag 121 gtcacccgca agggtggccg acgggcgaag aaacgacagg ctgaacagct gtccgcagca 181 ggagagggcg gggatgcggg ccgcatggac acagaggagg ccaggccggc gaagaggccc 241 gtcttcccac ccctctgtgg ggacgggctc ctGAGTGGGA AAGAAGAAAC AAGGAAAATT 301 CCAGTCCCAG CTAACAGATA CACACCATTG AAAGAAAACT GGATGAAGAT ATTTACTCCT 361 ATTGTGGAAC ATTTGGGACT TCAGATACGC TTTAACTTGA AATCAAGGAA TGTAGAAATC 421 AGGACTTGTA AAGAAACCAA GGATGTTAGT GCTCTGACAA AAGCAGCTGA TTTTGTGAAA 481 GCTTTTATTC TCGGCTTTCA GGTGGAGGAT GCACTTGCCC TCATCAGGTT GGATGACCTC 541 TTCCTAGAGT CTTTTGAAAT TACAGATGTT AAACCCCTAA AGGGAGACCA TCTATCCAGG 601 GCAATAGGAA GAATCGCTGG CAAAGGAGGA AAAACCAAAT TCACCATAGA GAATGTGACA 661 CGGACAAGGA TAGTTTTGGC TGATGTGAAA GTTCACATCC TTGGCTCCTT CCAAAATATC 721 AAGATGGCAA GAACTGCCAT TTGCAACCTA ATCTTGGGAA ATCCTCCTTC CAAGGTTTAT 781 GGCAATATTC GAGCTGTGGC TAGCAGATCA GCAGATCGAT TCTGCCCAAC TTTCTTGTAC 841 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 901 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 961 AAGGACGACG TCCGTAGTGT ATGAGATGCT TTACGCGTTA AGTCgacaat caacctctgg 1021 attacaaaat ttgtgaaaga tt