Construct: ORF TRCN0000474622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002457.1_s317c1
- Derived from:
- ccsbBroadEn_05589
- DNA Barcode:
- AATGCTTAAACCACGGCACACCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C1orf146 (388649)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474622
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388649 | C1orf146 | chromosome 1 open reading f... | NM_001012425.2 | 100% | 100% | |
| 2 | human | 388649 | C1orf146 | chromosome 1 open reading f... | XM_011541447.2 | 100% | 100% | |
| 3 | mouse | 76421 | 1700028K03Rik | RIKEN cDNA 1700028K03 gene | NM_182745.2 | 82.5% | 80.6% | (many diffs) |
| 4 | mouse | 76421 | 1700028K03Rik | RIKEN cDNA 1700028K03 gene | NM_175241.1 | 81.5% | 79.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 609
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgaaagt ggaaaagaaa aaataaaatg gacaaccacc attattatta 121 gctcatctct taagagttat GAAGTTGCAA CTGCCCTAGA AAATCGAAGC CACAAAGTTC 181 GATATTCAGA TTCAGTGGAA AATGGATCAA TTATATTTTC TCTTTCTGGA GTTGCATTTT 241 TATTAATGGA TACTAAGGAA TGTCTTCTGT CAACTGAAGA AATATTTCTA GCCAAAATTG 301 AGAAATTTAT TAACATTCAC CAAAATAGTT TTTTGGTTTT GTCTGCTGCC CTCCATGGGC 361 CTGAAGAATG GAAACTGATG TTCAGGATTC AGCAGAGATT CCTGGGTTGT AACTTACGAA 421 TACTTCCAGT ACACAACACA GTAAATGCTA TTAATCTTAT GTGCACTATA GCAAAGACTA 481 CCTCCAAACC ATACATAGAT AGCATTTGCT ACAGAATGAT AACAGCTAAA GCTTACATCA 541 TTGAGCAAAG TCCTGTTTGG AAAACACTTC AGAAGATAAA ACTGAATAGT GATTCAGTTA 601 ACCCAAATTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAATGCT TAAACCACGG CACACCATAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt