Construct: ORF TRCN0000474631
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006117.1_s317c1
- Derived from:
- ccsbBroadEn_03601
- DNA Barcode:
- CAAAAGTATCTTTAAGATTCCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIP4P2 (55529)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474631
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55529 | PIP4P2 | phosphatidylinositol-4,5-bi... | NM_018710.3 | 100% | 100% | |
| 2 | human | 55529 | PIP4P2 | phosphatidylinositol-4,5-bi... | XM_011517152.3 | 72.3% | 69.4% | (many diffs) |
| 3 | mouse | 72519 | Tmem55a | transmembrane protein 55A | NM_028264.4 | 90.5% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgctgatggg gtggacgaac gctcgcctct gctgtcagca tcccactccg 121 gaaatgtcac tcccaccgcc ccaccgtact tgcaagaaag cagccccaga gcggagctcc 181 cacctccata tacagccatt gccagtccag acgccagtgg tattccagta ataaactgcc 241 gtgtgtgcca atcactaatc aatttggatg gcaaGCTTCA CCAGCATGTG GTTAAGTGCA 301 CAGTTTGCAA TGAAGCTACG CCAATCAAAA ACCCCCCAAC AGGCAAGAAA TATGTTAGAT 361 GCCCTTGTAA TTGTCTTCTC ATTTGTAAGG ACACATCTCG GCGAATAGGA TGCCCAAGAC 421 CCAACTGTAG ACGGATAATT AACCTTGGCC CAGTAATGCT TATTTCTGAA GAACAACCAG 481 CTCAGCCTGC ATTGCCAATC CAACCAGAAG GTACAAGGGT CGTGTGTGGG CACTGTGGAA 541 ACACATTCCT GTGGATGGAA CTGAGGTTCA ACACTCTGGC AAAATGCCCA CACTGCAAAA 601 AAATCTCCTC AGTGGGTAGT GCACTTCCAC GAAGACGCTG CTGTGCATAT ATTACCATTG 661 GAATGATATG TATTTTCATT GGAGTTGGGT TAACTGTTGG CACCCCAGAT TTTGCAAGGC 721 GATTTCGAGC AACCTATGTT TCTTGGGCAA TTGCTTATCT CCTAGGATTG ATCTGCCTTA 781 TCCGAGCTTG TTATTGGGGA GCCATAAGAG TCAGTTATCC AGAACACAGT TTTGCATACC 841 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 901 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 961 ATATATCTTG TGGAAAGGAC GACAAAAGTA TCTTTAAGAT TCCAAAACGC GTTAAGTCga 1021 caatcaacct ctggattaca aaatttgtga aagatt