Construct: ORF TRCN0000474632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005212.1_s317c1
- Derived from:
- ccsbBroadEn_16097
- DNA Barcode:
- TAGCGTTTCGTGTGATGTGCTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFP28 (140612)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140612 | ZFP28 | ZFP28 zinc finger protein | NM_001308440.1 | 99.8% | 99.4% | 29G>A;971C>T |
| 2 | human | 140612 | ZFP28 | ZFP28 zinc finger protein | NM_020828.2 | 37.6% | 35.3% | (many diffs) |
| 3 | human | 140612 | ZFP28 | ZFP28 zinc finger protein | XM_011526463.3 | 32.8% | 28.6% | (many diffs) |
| 4 | human | 140612 | ZFP28 | ZFP28 zinc finger protein | XM_011526462.3 | 26.6% | 24.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gggggcggcg agcgcgagtg tccacgagcc gacgccgctc ccgggtagag 121 gcgccccccg cacaaagccc cgggcgggcc gaggcccgac tgtagggact ccagccacct 181 tggccctccc tgcccgggga aggccgcgct caaggaatgg cctcgcatcc aaaggccagc 241 gaggagcggc ccctacgggg cctgggcaca gagctctgcc ttccagggac actgctcttc 301 cccaggagag aaacaagaag ctggaggctg tggggacagg aattgaacct aaagccatgt 361 cccagggctt ggtgacattt ggggatgtgg ctgtagattt ctcccaagag gagtgggagt 421 ggctgaaccc cattcagagg aacttgtaca ggaaggtgat gttggagaac tacaggaacc 481 tggcatcgct gggactttgt gtttctaagc ccgatgtgat ctcctcgttg gaacaaggaa 541 aagagccttg gacagtgaag cgaaagatga caagagcctg gtgcccagac ttgaaggctg 601 tgtggaagat caaggagtta cctctcaaga aggacttctg cgaaggaaag ctatcccagg 661 cagtgataac agagagactc acaagctata atctggagta ctctctgtta gggGAACACT 721 GGGATTATGA TGCTCTGTTT GAGACACAGC CGGGCTTGGT GACTATCAAA AACCTGGCTG 781 TTGACTTCCG CCAGCAGCTA CACCCAGCTC AGAAGAATTT CTGTAAGAAT GGGATATGGG 841 AGAACAACAG TGACCTGGGA TCAGCAGGAC ATTGTGTGGC TAAGCCAGAT TTAGTCTCTT 901 TACTAGAGCA AGAGAAGGAG CCCTGGATGG TGAAGCGAGA GCTGACAGGA AGCCTGTTCT 961 CAGGTGAGTG CAGGAGAGCC TGGTGTGGAA AATCTACTGC AAGAGAGAGC GGAGCTTTTA 1021 CCAAAAAGGC TGCATTCTCA CTAATATACA GTAGGAAACT TTACCCAGGG TCTCTGGGTC 1081 TGGAAAGAAA ATTGAACTCT GGGAGTTCCA AATACCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1261 GCGTTTCGTG TGATGTGCTT TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt