Construct: ORF TRCN0000474633
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010375.1_s317c1
- Derived from:
- ccsbBroadEn_03386
- DNA Barcode:
- AATTTCAACTACACATACCGGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPA17 (53340)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474633
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53340 | SPA17 | sperm autoantigenic protein 17 | NM_017425.3 | 100% | 100% | |
| 2 | human | 53340 | SPA17 | sperm autoantigenic protein 17 | XM_011542870.2 | 100% | 100% | |
| 3 | human | 53340 | SPA17 | sperm autoantigenic protein 17 | XM_024448583.1 | 100% | 100% | |
| 4 | mouse | 20686 | Spa17 | sperm autoantigenic protein 17 | NM_001324545.1 | 81.8% | 70.8% | (many diffs) |
| 5 | mouse | 20686 | Spa17 | sperm autoantigenic protein 17 | NM_011449.2 | 81.8% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gattccattc tccaacaccc actaccgaat tccacaagga tttgggaatc 121 ttcttgaagg gctgacacgc gagattcTGA GAGAGCAACC GGACAATATA CCAGCTTTTG 181 CAGCAGCCTA TTTTGAGAGC CTTCTAGAGA AAAGAGAGAA AACCAACTTT GATCCAGCAG 241 AATGGGGGAG TAAGGTAGAA GACCGCTTCT ATAACAATCA TGCATTCGAG GAGCAAGAAC 301 CACCTGAGAA AAGTGATCCT AAACAAGAAG AGTCTCAGAT ATCTGGGAAG GAGGAAGAGA 361 CATCAGTCAC CATCTTAGAC TCTTCTGAGG AAGATAAGGA AAAAGAAGAG GTTGCTGCTG 421 TCAAAATCCA AGCTGCCTTC CGGGGACACA TAGCCAGAGA GGAGGCAAAG AAAATGAAAA 481 CAAATAGTCT TCAAAATGAG GAAAAAGAGG AAAACAAGTG CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGAAATTTC AACTACACAT ACCGGTACAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt