Construct: ORF TRCN0000474644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010599.1_s317c1
- Derived from:
- ccsbBroadEn_03659
- DNA Barcode:
- GGTAGCAAGTGCAGTCAGCTCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FOXJ2 (55810)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55810 | FOXJ2 | forkhead box J2 | NM_018416.3 | 100% | 100% | |
| 2 | human | 55810 | FOXJ2 | forkhead box J2 | XM_011520760.2 | 92.7% | 87.3% | 1537_1627del;1773_1774ins40 |
| 3 | human | 55810 | FOXJ2 | forkhead box J2 | XM_011520761.2 | 92.7% | 87.3% | 1537_1627del;1773_1774ins40 |
| 4 | human | 55810 | FOXJ2 | forkhead box J2 | XM_017019640.1 | 71.8% | 63.7% | (many diffs) |
| 5 | mouse | 60611 | Foxj2 | forkhead box J2 | NM_021899.3 | 87.6% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1791
- ORF length:
- 1722
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttctgac ctagagagta gcctcacctc catagactgg ctcccccagc 121 tgaccctccg agctaccatt gagaagcttg gaagtgcctc ccaggctggg cctcccggga 181 gcagccgcaa gtgttcacca gggtcaccca cagatcctaa tgccaccctg agcaaagacg 241 aggcagcagt gcaccaggac ggcaagccac gatacagcta tgccactctc atcacctatg 301 ccatcaactc ctctccagcc aagaagatga ccctcagcga gatttaccgc tggatctgtg 361 ataacttccc ctattacaag aatgctggca ttggttggaa gaattcaata cggcacaacc 421 tttctctcaa caagtgtttc cggaaggtgc ccagacctcg ggatgaccct gggaagggtt 481 cctattggac aattgacacc tgccctgaca tttcccgaaa gagaagacac cctccagatg 541 atgatctgtc ccaagactca ccagaacagg aggcaagcaa gagcccacgg ggaggcgttg 601 cagggagtgg agaagcctca ctgcctcctg aggggaatcc gcagatgtca cttcagagcc 661 ccacatctat agccagctac agccagggca caggatctgt ggatggtgga gcagtggcag 721 caggggcttc aggccgagaa agtgctgagg gtccccctcc cctctataac accaaccatg 781 actttaaatt ctcctactca gagatcaact ttcaggatct aagctggtcc ttccgcaacc 841 tctataagtc catgctggag aagtcctctt cctcctctca gcacggcttt tcttctctcc 901 tgggggacat cccaccctcg aacaactact acatgtatca gcagcagcag ccaccgccac 961 ctcaacagca gcagcagcag cagcagccgc cacagccacc tccccagcag tcccagccac 1021 agcagcagca ggcacctgcc cagggcccct cagctgtagg gggtgctcct ccactgcaca 1081 ccccaagcac agatggttgt accccaccag ggggaaagca agctggggcg gaaggctatg 1141 ggcctccccc tgtaatggcc atgcatccac ccccgctgca gcatggaggc taccaccctc 1201 atcagcacca tccccactcc caccctgccc agcagccacc acctccacag ccacaggcac 1261 aaggccaggc tcccatcaac aacactggct ttgcctttcc ttctgactgg tgctctaata 1321 ttgactcttt aaaggaaagc ttcaagatgg tgaatcggct caattggtcc agcattgagc 1381 agtcacaatt ctcagaactg atggagagtc tacgacaggc agagcagaag aactggaccc 1441 tcgaccagca tcacattgcc aatctgtgtg actcccTCAA CCACTTCCTT ACTCAGACTG 1501 GTCACGTGCC CCCTCAAGGG GGTACCCACC GCCCACCAGC CCCTGCCCGT ATTGCTGACT 1561 CCTGTGCCCT CACCAGTGGC AAACAGGAGT CAGCCATGAG CCAAGTGAAC TCTTATGGGC 1621 ACCCACAAGC TCCCCACCTC TACCCTGGCC CATCACCAAT GTACCCAATC CCCACCCAGG 1681 ACTCAGCAGG ATACAATCGC CCAGCACACC ATATGGTCCC TCGGCCATCA GTGCCACCTC 1741 CTGGTGCCAA TGAGGAGATC CCTGATGACT TCGACTGGGA CTTGATCACT TTGCCAACTT 1801 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1861 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1921 CTTGTGGAAA GGACGAGGTA GCAAGTGCAG TCAGCTCCGG ACGCGTTAAG TCgacaatca 1981 acctctggat tacaaaattt gtgaaagatt