Construct: ORF TRCN0000474647

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007395.1_s317c1
Derived from:
ccsbBroadEn_05682
DNA Barcode:
TATCGTAATACATCCCGTTGCTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CT45A5 (441521)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474647
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 102723631 CT45A10 cancer/testis antigen famil... NM_001291527.1 99.8% 100% 291C>T
2 human 102723631 CT45A10 cancer/testis antigen famil... NM_001291528.1 99.8% 100% 291C>T
3 human 102723631 CT45A10 cancer/testis antigen famil... NM_001291529.1 99.8% 100% 291C>T
4 human 102723631 CT45A10 cancer/testis antigen famil... NM_001291530.1 99.8% 100% 291C>T
5 human 102723737 CT45A8 cancer/testis antigen famil... NM_001291535.1 98% 94.7% (many diffs)
6 human 102723680 CT45A9 cancer/testis antigen famil... NM_001291540.2 98% 94.7% (many diffs)
7 human 102723680 CT45A9 cancer/testis antigen famil... NM_001321271.1 98% 94.7% (many diffs)
8 human 728911 CT45A2 cancer/testis antigen famil... NM_152582.7 98% 94.7% (many diffs)
9 human 728911 CT45A2 cancer/testis antigen famil... XM_006724783.2 98% 94.7% (many diffs)
10 human 102723680 CT45A9 cancer/testis antigen famil... XM_006724796.2 98% 94.7% (many diffs)
11 human 102723737 CT45A8 cancer/testis antigen famil... XM_006724799.2 98% 94.7% (many diffs)
12 human 102723737 CT45A8 cancer/testis antigen famil... XM_011531241.2 98% 94.7% (many diffs)
13 human 728911 CT45A2 cancer/testis antigen famil... XM_011531389.2 98% 94.7% (many diffs)
14 human 441521 CT45A5 cancer/testis antigen famil... NM_001007551.4 97.8% 94.7% (many diffs)
15 human 541465 CT45A6 cancer/testis antigen famil... NM_001017438.2 97.8% 94.7% (many diffs)
16 human 441521 CT45A5 cancer/testis antigen famil... NM_001172288.2 97.8% 94.7% (many diffs)
17 human 101060211 CT45A7 cancer/testis antigen famil... NM_001291543.2 97.8% 94.7% (many diffs)
18 human 101060211 CT45A7 cancer/testis antigen famil... XM_003960082.4 97.8% 94.7% (many diffs)
19 human 541465 CT45A6 cancer/testis antigen famil... XM_005262425.4 97.8% 94.7% (many diffs)
20 human 101060211 CT45A7 cancer/testis antigen famil... XM_011531229.3 97.8% 94.7% (many diffs)
21 human 441521 CT45A5 cancer/testis antigen famil... XM_011531341.3 97.8% 94.7% (many diffs)
22 human 541465 CT45A6 cancer/testis antigen famil... XM_011531351.2 97.8% 94.7% (many diffs)
23 human 441519 CT45A3 cancer/testis antigen famil... NM_001017435.2 97.7% 94.1% (many diffs)
24 human 441519 CT45A3 cancer/testis antigen famil... NM_001370148.2 97.7% 94.1% (many diffs)
25 human 441519 CT45A3 cancer/testis antigen famil... NM_001370149.1 97.7% 94.1% (many diffs)
26 human 541466 CT45A1 cancer/testis antigen famil... NM_001017417.2 97.5% 93.6% (many diffs)
27 human 541466 CT45A1 cancer/testis antigen famil... XM_005278141.4 97.5% 93.6% (many diffs)
28 human 541466 CT45A1 cancer/testis antigen famil... XM_011531352.1 97.5% 93.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
633
ORF length:
567
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac cgataaaaca gagaaggtgg ctgtagatcc tgaaactgtg tttaaacgtc
121ccagggaatg tgacagtcct tcgtatcaga aaaggcagag gatggccctg ttggcaagga
181aacaaggagc aggagacagc cttattgcag gcTCTGCCAT GTCCAAAGAA AAGAAGCTTA
241TGACAGGACA TGCTATTCCA CCCAGCCAAT TGGATTCTCA GATTGATGAC TTCACTGGTT
301TCAGCAAAGA TGGGATGATG CAGAAACCTG GTAGCAATGC ACCTGTGGGA GGAAATGTTA
361CCAGCAATTT CTCTGGAGAT GACCTAGAAT GCAGAGGAAT AGCCTCCTCT CCCAAAAGCC
421AACAAGAAAT TAATGCTGAT ATAAAATGTC AAGTAGTGAA GGAAATCCGA TGCCTTGGAC
481GAAAATATGA AAAAATCTTC GAAATGCTTG AAGGAGTGCA AGGACCTACT GCAGTCAGGA
541AACGATTTTT TGAATCCATC ATCAAGGAAG CAGCAAGATG TATGAGACGA GACTTTGTTA
601AGCACCTTAA GAAGAAACTG AAACGTATGA TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG
661ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
721GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA
781TCGTAATACA TCCCGTTGCT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat
841ttgtgaaaga tt

Download FASTA (ORF) (Full)