Construct: ORF TRCN0000474663
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016858.1_s317c1
- Derived from:
- ccsbBroadEn_06207
- DNA Barcode:
- AAATTTGTATGCCACCGTCGCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF6 (2251)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474663
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2251 | FGF6 | fibroblast growth factor 6 | NM_020996.2 | 99.5% | 100% | 189C>G;366T>C;417T>C |
| 2 | human | 2251 | FGF6 | fibroblast growth factor 6 | XM_017018995.1 | 76.2% | 69.2% | (many diffs) |
| 3 | human | 2251 | FGF6 | fibroblast growth factor 6 | XM_017018996.1 | 72.5% | 72.1% | (many diffs) |
| 4 | mouse | 14177 | Fgf6 | fibroblast growth factor 6 | NM_010204.1 | 88.4% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 693
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctggga cagaaactgt tcatcactat gtcccgggga gcaggacgtc 121 tgcagggcac gctgtgggct ctcgtcttcc taggcatcct agtgggcatg gtggtgccct 181 cgcctgcagg cacccgtgcc aacaacacgc tgctggactc gaggggctgg ggcaccctgc 241 tgtccaggtc tcgcgcgggg ctagctggag agattgccgg ggtgaactgg gaaagtggct 301 atttGGTGGG GATCAAGCGG CAGCGGAGGC TCTACTGCAA CGTGGGCATC GGCTTTCACC 361 TCCAGGTGCT CCCCGACGGC CGGATCAGCG GGACCCACGA GGAGAACCCC TACAGCCTGC 421 TGGAAATTTC CACCGTGGAG CGAGGCGTGG TGAGTCTCTT TGGAGTGAGA AGTGCCCTCT 481 TCGTCGCCAT GAACAGTAAA GGAAGATTGT ACGCAACGCC CAGCTTCCAA GAAGAATGCA 541 AGTTCAGAGA AACCCTCCTG CCCAACAATT ACAATGCCTA CGAGTCAGAC TTGTACCAAG 601 GGACCTACAT TGCCCTGAGC AAATACGGAC GGGTAAAGCG GGGCAGCAAG GTGTCCCCGA 661 TCATGACTGT CACTCATTTC CTTCCCAGGA TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 841 ATTTGTATGC CACCGTCGCC TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt