Transcript: Human NM_020996.2

Homo sapiens fibroblast growth factor 6 (FGF6), mRNA.

Source:
NCBI, updated 2019-05-04
Taxon:
Homo sapiens (human)
Gene:
FGF6 (2251)
Length:
744
CDS:
45..671

Additional Resources:

NCBI RefSeq record:
NM_020996.2
NBCI Gene record:
FGF6 (2251)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020996.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000038140 TGAGCAAATACGGACGGGTAA pLKO.1 592 CDS 100% 4.050 5.670 N FGF6 n/a
2 TRCN0000038139 GTAAAGGAAGATTGTACGCAA pLKO.1 472 CDS 100% 2.640 3.696 N FGF6 n/a
3 TRCN0000038141 GAACTGGGAAAGTGGCTATTT pLKO.1 260 CDS 100% 13.200 10.560 N FGF6 n/a
4 TRCN0000038142 CGAGGCGTGGTGAGTCTCTTT pLKO.1 417 CDS 100% 1.650 1.320 N FGF6 n/a
5 TRCN0000416040 GATCATGACTGTCACTCATTT pLKO_005 635 CDS 100% 13.200 9.240 N FGF6 n/a
6 TRCN0000038143 GCTGGAAATTTCCACTGTGGA pLKO.1 395 CDS 100% 2.640 1.848 N FGF6 n/a
7 TRCN0000416120 GAGTGAGAAGTGCCCTCTTCG pLKO_005 439 CDS 100% 1.350 0.945 N FGF6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020996.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06207 pDONR223 100% 99.5% 100% None 189C>G;366T>C;417T>C n/a
2 ccsbBroad304_06207 pLX_304 0% 99.5% 100% V5 189C>G;366T>C;417T>C n/a
3 TRCN0000474663 AAATTTGTATGCCACCGTCGCCTA pLX_317 61.6% 99.5% 100% V5 189C>G;366T>C;417T>C n/a
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