Construct: ORF TRCN0000474666

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008130.1_s317c1
Derived from:
ccsbBroadEn_06111
DNA Barcode:
ACAAAAGTCTGGCCGGTCAGATAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DYNC1I1 (1780)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474666
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1780 DYNC1I1 dynein cytoplasmic 1 interm... NM_001135556.1 99.8% 99.6% 622C>A;801G>T;1440C>A
2 human 1780 DYNC1I1 dynein cytoplasmic 1 interm... NM_004411.4 97.2% 97% (many diffs)
3 human 1780 DYNC1I1 dynein cytoplasmic 1 interm... NM_001135557.1 96.6% 96.4% (many diffs)
4 human 1780 DYNC1I1 dynein cytoplasmic 1 interm... NM_001278421.1 94.1% 93.9% (many diffs)
5 human 1780 DYNC1I1 dynein cytoplasmic 1 interm... NM_001278422.1 91.8% 89.5% (many diffs)
6 mouse 13426 Dync1i1 dynein cytoplasmic 1 interm... NM_010063.4 91.2% 97.7% (many diffs)
7 mouse 13426 Dync1i1 dynein cytoplasmic 1 interm... NM_001191026.1 89.5% 96% (many diffs)
8 mouse 13426 Dync1i1 dynein cytoplasmic 1 interm... XM_017321380.1 89.5% 96% (many diffs)
9 mouse 13426 Dync1i1 dynein cytoplasmic 1 interm... XM_006504982.3 88.8% 95.1% (many diffs)
10 mouse 13426 Dync1i1 dynein cytoplasmic 1 interm... NM_001191025.1 88.1% 94.5% (many diffs)
11 mouse 13426 Dync1i1 dynein cytoplasmic 1 interm... NM_001191027.1 86.4% 92.8% (many diffs)
12 mouse 13426 Dync1i1 dynein cytoplasmic 1 interm... NM_001191023.1 85.7% 92% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
67
ORF end:
1951
ORF length:
1884
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttgggcatgt ctgacaaaag tgacttaaaa gctgagctag agcgcaaaaa gcagcgctta
121gcacagataa gagaagagaa gaaacggaag gaagaggaga ggaaaaagaa agaggctgat
181atgcagcaga agaaagaacc cgttcaggac gactctgatc tggatcgcaa acgacgagag
241acagaggctt tgctgcaaag cattggtatc tcaccggagc cgcctctagt cccaacccct
301atgtctccct cctcgaaatc agtgagcact cccagtgaag ctggaagcca agactcaggc
361gatctggggc cattaacaag gaccctgcag tgggacacag acccctcagt gctccagctg
421cagtcagact cagaacttgg aagaagactg cataaactgg gcgtgtcaaa ggtcacccaa
481gtggatttcc tgccaaggga agtagtgtcc tactcaaagg agacccagac tcctcttgcc
541acgcatcagt ctgaagagga tgaggaagat gaggaaatgg tggaatctaa agttggccag
601gactcagaac tggaaaatca ggacaaaaaa caggaagtga aggaagcccc tccaagagag
661ttgacagagg aagaaaaaca gcagatcatt cattcagagg aatttctcat cttttttgac
721cggacaatac gggtaattga aagagccctg gctgaagatt ccgacatctt ttttgactac
781agcggccgag agttagagga aaaagatggg gatgttcagg ctggagccaa tctttctttc
841aatcgtcagt tctatgatga acattgttcc aagcatcgag tggtcacttg tatggactgg
901tccctccagt accctgagct gatggtggct tcttacaaca acaatgaaga tgctccccat
961gaaccagatg gagtggcctt ggtttggaac atgaagttta agaaaaccac accagaatac
1021gtcttccact gtcagtcctc tgtgatgtcg gtctgcttcg cccgtttcca tcctaacttg
1081gtggttggtg ggacttactc gggccagatt gtcctctggg acaatcgcag tcatcgaagg
1141actccagtgc agcggacacc cttatcagct gctgcacaca cgcatcccgt gtactgtgta
1201aatgttgttg ggacccagaa tgctcataac ctcatcactg tctccactga tggcaaaatg
1261tgttcctgga gcctggacat gctctcaact ccacaggaga gcatggagct ggtgtacaat
1321aagtccaagc ctgtcgctgt taccggaatg gctttcccaa cgggagacgt caataacttc
1381gtggttggca gtgaggaagg tacagtctac acggcttgTC GTCATGGAAG CAAAGCAGGT
1441ATTGGTGAGG TCTTTGAAGG TCACCAAGGG CCAGTGACAG GAATTAACTG CCACATGGCA
1501GTGGGACCAA TCGACTTTTC TCACCTGTTT GTCACATCAT CATTTGACTG GACTGTCAAA
1561CTGTGGACCA CCAAGCACAA CAAGCCGCTC TACTCCTTTG AAGACAATGC AGACTATGTG
1621TACGATGTCA TGTGGTCCCC CGTGCATCCT GCGCTTTTTG CCTGCGTGGA CGGGATGGGG
1681CGCTTGGACC TCTGGAACCT CAACAATGAC ACCGAGGTTC CAACAGCAAG TGTGGCCATT
1741GAGGGGGCAT CCGCCCTAAA CCGTGTTCGT TGGGCCCAAG CTGGCAAAGA AGTTGCTGTT
1801GGGGACTCGG AAGGCCGTAT TTGGGTCTAT GACGTTGGAG AGCTTGCAGT TCCCCACAAT
1861GATGAATGGA CCCGATTTGC CAGGACCCTT GTGGAAATTC GTGCTAACAG AGCTGATAGC
1921GAGGAGGAAG GCACTGTTGA GTTATCTGCC TACCCAACTT TCTTGTACAA AGTGGTTGAT
1981ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT
2041CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAA
2101AAGTCTGGCC GGTCAGATAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt
2161gtgaaagatt

Download FASTA (ORF) (Full)