Construct: ORF TRCN0000474666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008130.1_s317c1
- Derived from:
- ccsbBroadEn_06111
- DNA Barcode:
- ACAAAAGTCTGGCCGGTCAGATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC1I1 (1780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001135556.1 | 99.8% | 99.6% | 622C>A;801G>T;1440C>A |
| 2 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_004411.4 | 97.2% | 97% | (many diffs) |
| 3 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001135557.1 | 96.6% | 96.4% | (many diffs) |
| 4 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001278421.1 | 94.1% | 93.9% | (many diffs) |
| 5 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001278422.1 | 91.8% | 89.5% | (many diffs) |
| 6 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_010063.4 | 91.2% | 97.7% | (many diffs) |
| 7 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191026.1 | 89.5% | 96% | (many diffs) |
| 8 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | XM_017321380.1 | 89.5% | 96% | (many diffs) |
| 9 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | XM_006504982.3 | 88.8% | 95.1% | (many diffs) |
| 10 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191025.1 | 88.1% | 94.5% | (many diffs) |
| 11 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191027.1 | 86.4% | 92.8% | (many diffs) |
| 12 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191023.1 | 85.7% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 1951
- ORF length:
- 1884
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgggcatgt ctgacaaaag tgacttaaaa gctgagctag agcgcaaaaa gcagcgctta 121 gcacagataa gagaagagaa gaaacggaag gaagaggaga ggaaaaagaa agaggctgat 181 atgcagcaga agaaagaacc cgttcaggac gactctgatc tggatcgcaa acgacgagag 241 acagaggctt tgctgcaaag cattggtatc tcaccggagc cgcctctagt cccaacccct 301 atgtctccct cctcgaaatc agtgagcact cccagtgaag ctggaagcca agactcaggc 361 gatctggggc cattaacaag gaccctgcag tgggacacag acccctcagt gctccagctg 421 cagtcagact cagaacttgg aagaagactg cataaactgg gcgtgtcaaa ggtcacccaa 481 gtggatttcc tgccaaggga agtagtgtcc tactcaaagg agacccagac tcctcttgcc 541 acgcatcagt ctgaagagga tgaggaagat gaggaaatgg tggaatctaa agttggccag 601 gactcagaac tggaaaatca ggacaaaaaa caggaagtga aggaagcccc tccaagagag 661 ttgacagagg aagaaaaaca gcagatcatt cattcagagg aatttctcat cttttttgac 721 cggacaatac gggtaattga aagagccctg gctgaagatt ccgacatctt ttttgactac 781 agcggccgag agttagagga aaaagatggg gatgttcagg ctggagccaa tctttctttc 841 aatcgtcagt tctatgatga acattgttcc aagcatcgag tggtcacttg tatggactgg 901 tccctccagt accctgagct gatggtggct tcttacaaca acaatgaaga tgctccccat 961 gaaccagatg gagtggcctt ggtttggaac atgaagttta agaaaaccac accagaatac 1021 gtcttccact gtcagtcctc tgtgatgtcg gtctgcttcg cccgtttcca tcctaacttg 1081 gtggttggtg ggacttactc gggccagatt gtcctctggg acaatcgcag tcatcgaagg 1141 actccagtgc agcggacacc cttatcagct gctgcacaca cgcatcccgt gtactgtgta 1201 aatgttgttg ggacccagaa tgctcataac ctcatcactg tctccactga tggcaaaatg 1261 tgttcctgga gcctggacat gctctcaact ccacaggaga gcatggagct ggtgtacaat 1321 aagtccaagc ctgtcgctgt taccggaatg gctttcccaa cgggagacgt caataacttc 1381 gtggttggca gtgaggaagg tacagtctac acggcttgTC GTCATGGAAG CAAAGCAGGT 1441 ATTGGTGAGG TCTTTGAAGG TCACCAAGGG CCAGTGACAG GAATTAACTG CCACATGGCA 1501 GTGGGACCAA TCGACTTTTC TCACCTGTTT GTCACATCAT CATTTGACTG GACTGTCAAA 1561 CTGTGGACCA CCAAGCACAA CAAGCCGCTC TACTCCTTTG AAGACAATGC AGACTATGTG 1621 TACGATGTCA TGTGGTCCCC CGTGCATCCT GCGCTTTTTG CCTGCGTGGA CGGGATGGGG 1681 CGCTTGGACC TCTGGAACCT CAACAATGAC ACCGAGGTTC CAACAGCAAG TGTGGCCATT 1741 GAGGGGGCAT CCGCCCTAAA CCGTGTTCGT TGGGCCCAAG CTGGCAAAGA AGTTGCTGTT 1801 GGGGACTCGG AAGGCCGTAT TTGGGTCTAT GACGTTGGAG AGCTTGCAGT TCCCCACAAT 1861 GATGAATGGA CCCGATTTGC CAGGACCCTT GTGGAAATTC GTGCTAACAG AGCTGATAGC 1921 GAGGAGGAAG GCACTGTTGA GTTATCTGCC TACCCAACTT TCTTGTACAA AGTGGTTGAT 1981 ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT 2041 CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAA 2101 AAGTCTGGCC GGTCAGATAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt 2161 gtgaaagatt