Construct: ORF TRCN0000474683
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015923.1_s317c1
- Derived from:
- ccsbBroadEn_06908
- DNA Barcode:
- ATAGTACAAGTCGTTACCACGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- S100B (6285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474683
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6285 | S100B | S100 calcium binding protein B | NM_006272.3 | 99.6% | 100% | 99G>C |
| 2 | human | 6285 | S100B | S100 calcium binding protein B | XM_017028424.2 | 99.6% | 100% | 99G>C |
| 3 | mouse | 20203 | S100b | S100 protein, beta polypept... | NM_009115.3 | 85.1% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 181
- ORF end:
- 457
- ORF length:
- 276
- Sequence:
-
1 TGGAGACTGA AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT 61 TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC 121 CATTTCAGGT GTCGTGAGGC TAGCATCGAT TGATCAACAA GTTTGTACAA AAAAGTTGGC 181 ATGTCTGAGC TGGAGAAGGC CATGGTGGCC CTCATCGACG TTTTCCACCA ATATTCTGGA 241 AGGGAGGGAG ACAAGCACAA GCTGAAGAAA TCCGAACTCA AGGAGCTCAT CAACAATGAG 301 CTTTCCCATT TCTTAGAGGA AATCAAAGAG CAGGAGGTTG TGGACAAAGT CATGGAAACA 361 CTGGACAATG ATGGAGACGG CGAATGTGAC TTCCAGGAAT TCATGGCCTT TGTTGCCATG 421 GTTACTACTG CCTGCCACGA GTTCTTTGAA CATGAGTGCC CAACTTTCTT GTACAAAGTG 481 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 541 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 601 GAATAGTACA AGTCGTTACC ACGCCCACGC GTTAAGTCga caatcaacct ctggattaca 661 aaatttgtga aagatt