Construct: ORF TRCN0000474684
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006920.1_s317c1
- Derived from:
- ccsbBroadEn_14257
- DNA Barcode:
- AGATGTAGTTGTAGGTTCGTCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMIM7 (79086)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474684
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79086 | SMIM7 | small integral membrane pro... | XR_244083.3 | 13.1% | (many diffs) | |
| 2 | human | 79086 | SMIM7 | small integral membrane pro... | XR_244086.4 | 10.1% | (many diffs) | |
| 3 | human | 79086 | SMIM7 | small integral membrane pro... | XR_244084.4 | 10% | (many diffs) | |
| 4 | human | 79086 | SMIM7 | small integral membrane pro... | XR_001753760.2 | 9.8% | (many diffs) | |
| 5 | human | 79086 | SMIM7 | small integral membrane pro... | XR_001753759.2 | 8.8% | 1_655del;675C>T;809_1717del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 280
- ORF end:
- 433
- ORF length:
- 153
- Sequence:
-
1 GTCCAGGCAC CTCGATTAGT TCTCGAGCTT TTGGAGTACG TCGTCTTTAG GTTGGGGGGG 61 AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG 121 CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA 181 TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT 241 AGCATCGATT GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCGAGTCT CCATTCCTTC 301 ATTCCTGATG ACTTCAAGAA TGTTTTTGAC CAGAAAACCG ACAACCTTCC CAGAAAGTCC 361 AAGCTCGTGG TGGGTGGAAA AGTGTTCGCC GAGGTGTGCA TGGTTTCCCA GCCACGTCCC 421 TGTTTTCAAA GATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT 481 AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT 541 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG ATGTAGTTGT AGGTTCGTCA 601 TGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt