Construct: ORF TRCN0000474687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013374.1_s317c1
- Derived from:
- ccsbBroadEn_12558
- DNA Barcode:
- ACGTTTCTACGGTGTCCTGTTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM43 (79188)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79188 | TMEM43 | transmembrane protein 43 | XM_017007176.2 | 62.4% | 62.4% | 1_411del |
| 2 | human | 79188 | TMEM43 | transmembrane protein 43 | NM_024334.2 | 57% | 57% | 1_516del |
| 3 | mouse | 74122 | Tmem43 | transmembrane protein 43 | NM_028766.3 | 50.1% | 52.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 750
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agtggagtca ttcatggcaa cagccccctt tgtccaaatt ggcaggtttt 121 tcctctcgtc aggcctcatc gacaaagtcg acaacttcaa gtccctgagc ctatccaagc 181 tggaggaccc TCATGTGGAC ATCATTCGCC GTGGAGACTT TTTCTACCAC AGCGAAAATC 241 CCAAGTATCC AGAGGTGGGA GACTTGCGTG TCTCCTTTTC CTATGCTGGA CTGAGCGGCG 301 ATGACCCTGA CCTGGGCCCA GCTCACGTGG TCACTGTGAT TGCCCGGCAG CGGGGTGACC 361 AGCTAGTCCC ATTCTCCACC AAGTCTGGGG ATACCTTACT GCTCCTGCAC CACGGGGACT 421 TCTCAGCAGA GGAGGTGTTT CATAGAGAAC TAAGGAGCAA CTCCATGAAG ACCTGGGGCC 481 TGCGGGCAGC TGGCTGGATG GCCATGTTCA TGGGCCTCAA CCTTATGACA CGGATCCTCT 541 ACACCTTGGT GGACTGGTTT CCTGTTTTCC GAGACCTGGT CAACATTGGC CTGAAAGCCT 601 TTGCCTTCTG TGTGGCCACC TCGCTGACCC TGCTGACCGT GGCGGCTGGC TGGCTCTTCT 661 ACCGACCCCT GTGGGCCCTC CTCATTGCCG GCCTGGCCCT TGTGCCCATC CTTGTTGCTC 721 GGACACGGGT GCCAGCCAAA AAGTTGGAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACGTT 901 TCTACGGTGT CCTGTTTAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 961 tgaaagatt