Construct: ORF TRCN0000474704
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005252.1_s317c1
- Derived from:
- ccsbBroadEn_14264
- DNA Barcode:
- CGGACGCACTCTTTTGGTGATCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNASEH2B (79621)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474704
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79621 | RNASEH2B | ribonuclease H2 subunit B | NM_024570.4 | 92.8% | 91.5% | 616G>A;908_914delTAAAAAA;936_937ins64 |
| 2 | human | 79621 | RNASEH2B | ribonuclease H2 subunit B | XM_006719867.4 | 87.5% | 86.1% | (many diffs) |
| 3 | human | 79621 | RNASEH2B | ribonuclease H2 subunit B | XM_011535229.1 | 78.1% | 73% | (many diffs) |
| 4 | human | 79621 | RNASEH2B | ribonuclease H2 subunit B | NM_001142279.2 | 76.8% | 72.6% | (many diffs) |
| 5 | human | 79621 | RNASEH2B | ribonuclease H2 subunit B | XM_011535231.2 | 76.8% | 75.2% | (many diffs) |
| 6 | human | 79621 | RNASEH2B | ribonuclease H2 subunit B | XM_011535230.2 | 75.6% | 72.6% | (many diffs) |
| 7 | human | 79621 | RNASEH2B | ribonuclease H2 subunit B | XM_017020747.1 | 75% | 73% | (many diffs) |
| 8 | human | 79621 | RNASEH2B | ribonuclease H2 subunit B | XM_011535233.2 | 50.9% | 48.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgctggcgtg gactgcgggg acggggttgg cgcccggcag cacgtgttcc 121 tggtttcaga atatttaaaa gatgcttcaa agaagatgaa aaatgggcta atgtttgtaa 181 aactggttaa cccctgttca ggagaaggag ccatttactt gttcaatatg tgtctacagc 241 agctgtttga agtaaaagtt ttcaaggaaa aacaccattc ttggtttata aatcaatcag 301 ttcaatcagg aggtcttctc cattttgcca cacctgtgga tcctctattt ctgcttctcc 361 actacctcat aaaggctgat aaggagggga agtttcagcc ccttgatcaa gttgtggtgg 421 ataacgtgtt tccaaattgc atcttgttgc tgaaacttcc tggacttgag aagttacttc 481 atcatgtgac agaggaaaaa ggtaatccag aaatagacaa caagaaatat tacaagtaca 541 gcaaagagaa gacattaaag tggctggaaa aaaaggttaa tcaaactgtg gcagcattaa 601 aaaccaataa tgtgaatgtc agttcccggg tacagtcaac tgcatttttc tctggtgacc 661 aagcttccac tgacaaggaa aaggattata ttcgttatgc ccatggtctg atatctgact 721 acatccctaa agaattaagt gatgacttat ctaaatactt aaagcttcca gaaccttcag 781 cctcattgcc aaaTCCTCCA TCAAAGAAAA TAAAGTTATC AGATGAGCCT GTAGAAGCAA 841 AAGAAGATTA CACTAAGTTT AATACTAAAG ATTTGAAGAC TGAAAAGAAA AATAGCAAAA 901 TGACTGCAGC TCAGAAGGCT TTGGCTAAAG TTGACAAGAG TGGAATGAAA AGTATTGATA 961 CCTTTTTTGG GGTAAAAAAA AAATTGGAAA GGTTTGAAAC TTTGAAAATA AAATCTAGCA 1021 AAAATATTTG CTTTTTACAT GTTTCAGTTT GTCCTTACTG CCCGACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGACGGACG CACTCTTTTG GTGATCTTAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt