Construct: ORF TRCN0000474708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013647.1_s317c1
- Derived from:
- ccsbBroadEn_06355
- DNA Barcode:
- CGATCAAGAACGGCAATCTTGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HCLS1 (3059)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474708
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3059 | HCLS1 | hematopoietic cell-specific... | NM_005335.6 | 99.7% | 99.5% | 441C>T;703A>G;1306G>C |
| 2 | human | 3059 | HCLS1 | hematopoietic cell-specific... | NM_001292041.2 | 92.1% | 91.9% | (many diffs) |
| 3 | mouse | 15163 | Hcls1 | hematopoietic cell specific... | NM_008225.2 | 83.5% | 83.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1524
- ORF length:
- 1458
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gaagtctgta gtgggccatg atgtgtctgt ttccgtggag acccagggtg 121 atgattggga cacagatcct gactttgtga atgacatctc tgaaaaggag caacgatggg 181 gagccaagac catcgagggg tctggacgca cagaacacat caacatccac cagctgagga 241 acaaagtatc agaggagcat gatgttctca ggaagaaaga gatggagtca gggcccaaag 301 catcccatgg ctatggaggt cggtttggag tagaaagaga ccgaatggac aagagtgcag 361 tgggccatga gtatgttgcc gaggtggaga agcactcttc tcagacggat gctgccaaag 421 gctttggggg caagtacgga gttgagaggg acagggcaga caagtcagca gtcggctttg 481 attataaagg agaagtggag aagcatacat ctcagaaaga ttactctcgt ggctttggtg 541 gccggtacgg ggtggagaag gataaatggg acaaagcagc tctgggatat gactacaagg 601 gagagacgga gaaacacgag tcccagagag attatgccaa gggctttggt ggccagtatg 661 gaatccagaa ggaccgagtg gataagagcg ctgtcggctt caatgaaatg gaggccccga 721 ccacagctta taagaagacg acgcccatag aagccgcttc tagtggtgcc cgtgggctga 781 aggcgaaatt tgagtccatg gctgaggaga agaggaagcg agaggaagag gagaaggcac 841 agcaggtggc caggaggcaa caggagcgaa aggctgtgac aaagaggagc cctgaggctc 901 cacagccagt gatagctatg gaagagccag cagtaccggc cccactgccc aagaaaatct 961 cctcagaggc ctggcctcca gttgggactc cTCCATCATC AGAGTCTGAG CCTGTGAGAA 1021 CCAGCAGGGA ACACCCAGTG CCCTTGCTGC CCATTAGGCA GACTCTCCCG GAGGACAATG 1081 AGGAGCCCCC AGCTCTGCCC CCTAGGACTC TGGAAGGCCT CCAGGTGGAG GAAGAGCCAG 1141 TGTACGAAGC AGAGCCTGAG CCTGAGCCCG AGCCTGAGCC CGAGCCTGAG AATGACTATG 1201 AGGACGTTGA GGAGATGGAC AGGCATGAGC AGGAGGATGA ACCAGAGGGG GACTATGAGG 1261 AGGTGCTCGA GCCTGAAGAT TCTTCTTTTT CTTCTGCTCT GGCTGGATCA TCAGGCTGCC 1321 CGGCTGGGGC TGGGGCTGGG GCTGTGGCTC TGGGGATCTC AGCTGTGGCT CTATATGATT 1381 ACCAAGGAGA GGGAAGTGAT GAGCTTTCCT TTGATCCGGA CGACGTAATC ACTGACATTG 1441 AGATGGTGGA CGAGGGCTGG TGGCGGGGAC GTTGCCATGG CCACTTTGGA CTCTTCCCTG 1501 CAAATTATGT CAAGCTTCTG GAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1561 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1621 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GATCAAGAAC 1681 GGCAATCTTG AACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1741 att