Construct: ORF TRCN0000474717
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003455.1_s317c1
- Derived from:
- ccsbBroadEn_13359
- DNA Barcode:
- TGTGACCTCCGTAGTTACCAAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PWWP2B (170394)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474717
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 170394 | PWWP2B | PWWP domain containing 2B | NM_138499.4 | 86.8% | 86.6% | 1_231del;265C>T;292C>G |
| 2 | human | 170394 | PWWP2B | PWWP domain containing 2B | XM_006717659.3 | 86.8% | 86.6% | 1_231del;265C>T;292C>G |
| 3 | human | 170394 | PWWP2B | PWWP domain containing 2B | XM_011539387.2 | 75.8% | 70.3% | (many diffs) |
| 4 | human | 170394 | PWWP2B | PWWP domain containing 2B | NM_001098637.2 | 70.8% | 70.6% | (many diffs) |
| 5 | human | 170394 | PWWP2B | PWWP domain containing 2B | XR_001747051.1 | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1605
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gctggggtcc agctcccccc ctcctgcctg cggggttcag ccccccgaga 121 ccaccggccc cgagccaccc ccgcccctcg tgccgccgct gcccgccgga agcctgcccc 181 cgtaccctcc ctacttcgaa ggcgccccct tccctcaccc gctgtggctc cgggacacgt 241 acaagctgtg ggtgccccag ccgccgccca ggaccatcaa gcgcacgcgg cggcgtctgt 301 cccgcaaccg cgacccgggg cgcctcatcc tcagcaccat ccgcctgcgg ccgcgccagg 361 tgctctgtga gaagtgcaag agcacgctga gccccccgga ggccagcccc ggacccccag 421 ccgcgcccag ggcccgcagg aggctgggca gcggcccgga cagggagctc cgcaagccgg 481 aggagccgga gaacggcgag cccacggctg cggccaccgc caggaggagc aagagggaga 541 ggcgcgagga ggacagggcc ccggcagagc aggtcccgcg gagcccggtc atcaagatct 601 cctacagcac gccccagggc aagggagagg tggtcaagat cccctcccgc gtgcacggct 661 ctctggagcc cttccgtccc cagcaggccc cgcaggacga cggcagccag gaccccgagg 721 tgctggacag agagtcccgg gaccggccgt cctgcgcgcc ctcggcctcc atccccaagt 781 tgaaactgac acggcctgtg ccggccggcg cggacctgcc gccccctaag atccgcctga 841 agccccaccg tctgggggac agcgagcacg agcccgtgta ccgggccgag ctggtggggg 901 agctgaacgg gtacctgcgg gacagctcgc cggcgccctg tgcggacggc cctgccggtg 961 ggctggcgga cttgtcttct ggaagttcgg gtgaggacga tgacttcaag agctgtcccc 1021 agggtccaca gggacgcgag ggcttggctt ttctcgtcag ctgccctgag gggagagcgg 1081 actgtgccag tgagtcggcg tgcagcagcg acagcctgga cgaggccaga tcgtccggct 1141 cggaagggac gccggcagac acgggtgaCC TCTCGCCTGG CCACGGCGCG TCAGCGCCCT 1201 CGGTGTCCAG AGAGGCTCGC CAAACGGTGC CGCCCCTGAC GGTCAGGCTG CACACACAGA 1261 GCGTGTCGGA GTGCATCACG GAGGACGGCA GGACTGTGGC CGTGGGGGAC ATCGTCTGGG 1321 GTAAGATCCA TGGTTTTCCT TGGTGGCCGG CGCGTGTTCT TGACATCAGT CTCGGCCAGA 1381 AGGAGGACGG AGAGCCGTCT TGGCGAGAAG CGAAGGTCTC GTGGTTTGGT TCTCCGACTA 1441 CGTCGTTCTT GTCTATTTCA AAACTCTCCC CTTTCTCTGA ATTTTTCAAA CTGAGATTTA 1501 ATCGTAAGAA GAAGGGGATG TATCGGAAAG CTATAACCGA GGCTGCAAAT GCCGCAAGAC 1561 ACGTGGCCCC GGAAATCAGG GAGCTCTTAA CCCAGTTTGA AACGTACCCA ACTTTCTTGT 1621 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1681 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1741 GAAAGGACGA TGTGACCTCC GTAGTTACCA AATGACGCGT TAAGTCgaca atcaacctct 1801 ggattacaaa atttgtgaaa gatt