Construct: ORF TRCN0000474727
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015392.1_s317c1
- Derived from:
- ccsbBroadEn_09800
- DNA Barcode:
- CGTGGCCTGTGACTCCAGGGAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG14 (199857)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474727
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 199857 | ALG14 | ALG14 UDP-N-acetylglucosami... | NM_144988.4 | 99.8% | 99.5% | 47T>N |
| 2 | human | 199857 | ALG14 | ALG14 UDP-N-acetylglucosami... | XM_005270582.4 | 69.5% | 65.7% | (many diffs) |
| 3 | human | 199857 | ALG14 | ALG14 UDP-N-acetylglucosami... | XM_011540897.2 | 66.7% | 61.1% | (many diffs) |
| 4 | human | 199857 | ALG14 | ALG14 UDP-N-acetylglucosami... | NM_001305242.2 | 62.3% | 47.1% | 47T>N;288_324del;465_466ins220 |
| 5 | human | 199857 | ALG14 | ALG14 UDP-N-acetylglucosami... | XR_946568.3 | 58% | (many diffs) | |
| 6 | human | 199857 | ALG14 | ALG14 UDP-N-acetylglucosami... | XR_001737024.1 | 54.7% | (many diffs) | |
| 7 | human | 199857 | ALG14 | ALG14 UDP-N-acetylglucosami... | XR_001737025.1 | 26.1% | (many diffs) | |
| 8 | human | 199857 | ALG14 | ALG14 UDP-N-acetylglucosami... | NR_131032.2 | 5.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gtgcgttctc gttctagctg cggccgcagg agctgtggcg gntttcctaa 121 tcctgcgaat atgggtagtg cttcgttcca tggacgttac gccccgggag tctctcagta 181 tcttggtagt ggctgggtcc ggtgggcata ccactgagat cctgaggctg cttgggagct 241 tgtccaatgc ctacTCACCT AGACATTATG TCATTGCTGA CACTGATGAA ATGAGTGCCA 301 ATAAAATAAA TTCTTTTGAA CTAGATCGAG CTGATAGAGA CCCTAGTAAC ATGTATACCA 361 AATACTACAT TCACCGAATT CCAAGAAGCC GGGAGGTTCA GCAGTCCTGG CCCTCCACCG 421 TTTTCACCAC CTTGCACTCC ATGTGGCTCT CCTTTCCCCT AATTCACAGG GTGAAGCCAG 481 ATTTGGTGTT GTGTAACGGA CCAGGAACAT GTGTTCCTAT CTGTGTATCT GCCCTTCTCC 541 TTGGGATACT AGGAATAAAG AAAGTGATCA TTGTCTACGT TGAAAGCATC TGCCGTGTAG 601 AAACGTTATC CATGTCCGGA AAGATTCTGT TTCATCTCTC AGATTACTTC ATTGTTCAGT 661 GGCCGGCTCT GAAAGAAAAG TATCCCAAAT CGGTGTACCT TGGGCGAATT GTTTGCCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAC GTGGCCTGTG ACTCCAGGGA ATAACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att